Rabbit SDR Polyclonal Antibody | anti-SDR antibody

SDR, ID (SDPR, Serum deprivation-response protein, Cavin-2, PS-p68, Phosphatidylserine-binding protein) (Biotin)

Cat. Num. AAA6345648

• Gene Names: SDPR; SDR; CAVIN2; PS-p68; cavin-2
• Reactivity: Human, Hamster
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: SDR; Polyclonal Antibody; ID (SDPR; Serum deprivation-response protein; Cavin-2; PS-p68; Phosphatidylserine-binding protein) (Biotin); SDPR; Phosphatidylserine-binding protein; anti-SDR antibody

• Host: Rabbit
• Reactivity: Human, Hamster
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SDR. Species Crossreactivity: Hamster
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SDR antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: SDR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115~145 amino acids from the Central region of human SDR.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SDR Antibody (Center) in CHO cell line lysates (35ug/lane). SDR (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(SDR Antibody (Center) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SDR Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SDR Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SDR antibody

This gene encodes a calcium-independent phospholipid-binding protein whose expression increases in serum-starved cells. This protein is a substrate for protein kinase C (PKC) phosphorylation and recruits polymerase I and transcript release factor (PTRF) to caveolae. Removal of this protein causes caveolae loss and its over-expression results in caveolae deformation and membrane tubulation.

Product Categories/Family for anti-SDR antibody

Antibodies; Binding Proteins

References

Baig, A., et al. Proteomics 9(17):4254-4258(2009). Hansen, C.G., et al. Nat. Cell Biol. 11(7):807-814(2009). Ogata, T., et al. Mol. Cell. Biol. 28(10):3424-3436(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4759082
• NCBI GeneID: 8436
• NCBI Accession #: NP_004648
• NCBI GenBank Nucleotide #: NM_004657.5
• UniProt Accession #: O95810
• Molecular Weight: 47 kDa
• NCBI Official Full Name: serum deprivation-response protein
• NCBI Official Synonym Full Names: serum deprivation response
• NCBI Official Symbol: SDPR
• NCBI Official Synonym Symbols: SDR; CAVIN2; PS-p68; cavin-2
• NCBI Protein Information: serum deprivation-response protein; SDPR; phosphatidylserine binding protein; phosphatidylserine-binding protein
• UniProt Protein Name: Serum deprivation-response protein
• Protein Family: Tropinone reductase
• UniProt Gene Name: SDPR
• UniProt Entry Name: SDPR_HUMAN

NCBI Description

This gene encodes a calcium-independent phospholipid-binding protein whose expression increases in serum-starved cells. This protein is a substrate for protein kinase C (PKC) phosphorylation and recruits polymerase I and transcript release factor (PTRF) to caveolae. Removal of this protein causes caveolae loss and its over-expression results in caveolae deformation and membrane tubulation.[provided by RefSeq, Sep 2009]

Uniprot Description

SDPR: May play a role in targeting PRKCA to caveolae. Belongs to the PTRF/SDPR family.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 2q32-q33

Cellular Component: cytoplasm; caveola; cytosol

Molecular Function: protein binding; protein kinase C binding; phosphatidylserine binding; phospholipid binding