Ribonuclease A ELISA Pair Set | RNASE1 elisa pair set

Ribonuclease A Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118450

• Synonyms: Ribonuclease A; Ribonuclease A Matched ELISA Antibody Pair Set; Human; Human Ribonuclease A/RNASE1 ELISA Pair Set; ribonuclease; RNase A family; 1 (pancreatic); RAC1 Matched ELISA Antibody Pair Set; RIB1 Matched ELISA Antibody Pair Set; RNS1 Matched ELISA Antibody Pair Set; RNASE1 elisa pair set

• Specificity: Quantitative determination of Human Ribonuclease A

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 62.5-4000pg/mL
• Sensitivity: 62.5pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of mouse anti-Human Ribonuclease A/RNASE1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 1ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human Ribonuclease A/RNASE1 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.25ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 85 ng of recombinant Human Ribonuclease A/RNASE1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for RNASE1 elisa pair set

RNase A, also known as ribonuclease A and RNASE1, belongs to ribonuclease A superfamily. It is a pancreatic-type of secretory ribonuclease. RNase A is a basic protein and its many positive charges are consistent with its binding to RNA (a poly-anion). More generally, RNase A is unusually polar or, rather, unusually lacking in hydrophobic groups, especially aliphatic ones. As an endonuclease, RNase A cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2', 3'-cyclic nucleotides, with a pH optimum near 8.0. RNase A is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

This Ribonuclease A Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Ribonuclease A. It contains Human Ribonuclease A capture antibody, Human Ribonuclease A detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Ribonuclease A protein.

References

Tubert P, et al. (2011) Interactions crucial for three-dimensional domain swapping in the HP-RNase variant PM8. Biophys J. 101(2):459-67.Vinayagam A, et al. (2011) A directed protein interaction network for investigating intracellular signal transduction. Sci Signal. 4(189):rs8.Fischer S, et al. (2011) Expression and localisation of vascular ribonucleases in endothelial cells. Thromb Haemost. 105(2):345-55.