Surfactant protein D/SFTPD ELISA Pair Set | SFTPD elisa pair set

Surfactant protein D/SFTPD Matched ELISA Antibody Pair Set, Mouse

Cat. Num. AAA8118482

• Synonyms: Surfactant protein D/SFTPD; Surfactant protein D/SFTPD Matched ELISA Antibody Pair Set; Mouse; Mouse SFTPD/SP-D/SFTP4 ELISA Pair Set; surfactant protein D; AI573415 Matched ELISA Antibody Pair Set; Sftp4 Matched ELISA Antibody Pair Set; SP-D Matched ELISA Antibody Pair Set; SFTPD elisa pair set

• Specificity: Quantitative determination of Mouse Surfactant protein D/SFTPD

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 62.5-4000pg/mL
• Sensitivity: 62.5pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of rabbit anti-Mouse SFTPD/SP-D/SFTP4 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse SFTPD/SP-D/SFTP4 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.6ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 105 ng of recombinant Mouse SFTPD/SP-D/SFTP4. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for SFTPD elisa pair set

Surfactant pulmonary-associated protein D, also known as SFTPD and SP-D, is a member of the collectin family of C-type lectins that is synthesized in many tissues including respiratory epithelial cells in the lung, and contains one C-type lectin domain and one collagen-like domain. The polymorphic variation in the N-terminal domain of the SP-D molecule influences oligomerization, function, and the concentration of the molecule in serum. SFTPD is produced primarily by alveolar type II cells and nonciliated bronchiolar cells in the lung and is constitutively secreted into the alveoli where it influences surfactant homeostasis, effector cell functions, and host defense. It is upregulated in a variety of inflammatory and infectious conditions including Pneumocystis pneumonia and asthma. SFTPD is humoral molecules of the innate immune system, and is considered a functional candidate in chronic periodontitis. Besides, it is involved in the development of acute and chronic inflammation of the lung. Several human lung diseases are characterized by decreased levels of bronchoalveolar SFTPD. Thus, recombinant SFTPD has been proposed as a therapeutical option for cystic fibrosis, neonatal lung disease and smoking-induced emphysema. Furthermore, SFTPD serum levels can be used as disease activity markers for interstitial lung diseases.

This Surfactant protein D/SFTPD Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse Surfactant protein D/SFTPD. It contains Mouse Surfactant protein D/SFTPD capture antibody, Mouse Surfactant protein D/SFTPD detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse Surfactant protein D/SFTPD protein.

References

Leth-Larsen R, et al. (2005) A common polymorphism in the SFTPD gene influences assembly, function, and concentration of surfactant protein D. J Immunol. 174(3): 1532-8.Moran AP, et al. (2005) Role of surfactant protein D (SP-D) in innate immunity in the gastric mucosa: evidence of interaction with Helicobacter pylori lipopolysaccharide. J Endotoxin Res. 11(6): 357-62.Hartl D, et al. (2006) Surfactant protein D in human lung diseases. Eur J Clin Invest. 36(6): 423-35.Krueger M, et al. (2006) Amino acid variants in Surfactant protein D are not associated with bronchial asthma. Pediatr Allergy Immunol. 17(1): 77-81.Glas J, et al. (2008) Increased plasma concentration of surfactant protein D in chronic periodontitis independent of SFTPD genotype: potential role as a biomarker. Tissue Antigens. 72(1): 21-8.