Ribonuclease A Recombinant Protein | RNASE1 recombinant protein

Ribonuclease A Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8118684

• Gene Names: RNASE1; RAC1; RIB1; RNS1
• Purity: >95 % as determined by SDS-PAGE
• Synonyms: Ribonuclease A; Ribonuclease A Protein; Human; Recombinant (His Tag); Human RNase A/Ribonuclease A/RNASE1 Protein (His Tag); ribonuclease; RNase A family; 1 (pancreatic); RAC1 Protein; RIB1 Protein; RNS1 Protein; RNASE1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 139aa
• Sequence: Met1-Thr156

• Species: Human
• Tag: C-His
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Lys29
• Protein Construction: |A DNA sequence encoding the human RNASE1 (P07998) (Met1-Thr156) was expressed with a polyhistidine tag at the C-terminus
• Activity: Measured by its ability to hydrolyze yeast RNA (Sigma). One unit of the enzyme causes an increase in absorbance of 1.0 at 260 nm in 15 min when yeast RNA is hydrolyzed at 37 degree C and pH7.0.; The specific activity is >2.5x106 unit/mg.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Related Product Information for RNASE1 recombinant protein

RNase A, also known as ribonuclease A and RNASE1, belongs to ribonuclease A superfamily. It is a pancreatic-type of secretory ribonuclease. RNase A is a basic protein and its many positive charges are consistent with its binding to RNA (a poly-anion). More generally, RNase A is unusually polar or, rather, unusually lacking in hydrophobic groups, especially aliphatic ones. As an endonuclease, RNase A cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2', 3'-cyclic nucleotides, with a pH optimum near 8.0. RNase A is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

References

Tubert P, et al. (2011) Interactions crucial for three-dimensional domain swapping in the HP-RNase variant PM8. Biophys J. 101(2):459-67.Vinayagam A, et al. (2011) A directed protein interaction network for investigating intracellular signal transduction. Sci Signal. 4(189):rs8.Fischer S, et al. (2011) Expression and localisation of vascular ribonucleases in endothelial cells. Thromb Haemost. 105(2):345-55.

NCBI and Uniprot Product Information

• NCBI GI #: 4506547
• NCBI GeneID: 6035
• NCBI Accession #: NP_002924.1
• NCBI GenBank Nucleotide #: NM_002933.5
• UniProt Accession #: P07998; Q16830; Q16869; Q1KHR2; Q6ICS5; Q9UCB4; Q9UCB5; B2R589; D3DS06
• Molecular Weight: 17,644 Da
• NCBI Official Full Name: ribonuclease pancreatic
• NCBI Official Synonym Full Names: ribonuclease A family member 1, pancreatic
• NCBI Official Symbol: RNASE1
• NCBI Official Synonym Symbols: RAC1; RIB1; RNS1
• NCBI Protein Information: ribonuclease pancreatic
• UniProt Protein Name: Ribonuclease pancreatic
• Protein Family: Ribonuclease
• UniProt Gene Name: RNASE1
• UniProt Synonym Gene Names: RIB1; RNS1; RNase 1; RNase A

NCBI Description

This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA.