CEACAM5 elisa pair set

CEACAM5 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118348

• Synonyms: CEACAM5; CEACAM5 Matched ELISA Antibody Pair Set; Human; Human CEACAM5/CD66e ELISA Pair Set; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e Matched ELISA Antibody Pair Set; CEA Matched ELISA Antibody Pair Set; CEACAM5 elisa pair set

• Specificity: Quantitative determination of Human CEACAM5

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 62.5-4000pg/mL
• Sensitivity: 62.5pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of mouse anti-Human CEACAM5/CD66e monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of mouse anti-Human CEACAM5/CD66e monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.3ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 70 ng of recombinant Human CEACAM5/CD66e. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for CEACAM5 elisa pair set

CEACAM5, also known as CEA or D66e, belongs to the large CEACAM subfamily of the immunoglobulin superfamily. CEACAM5 is expressed primarily by epithelial cells, and is synthesized as a glycoprotein with an MW of 180 kDa comprising 60% carbohydrate. CEACAM5 contains one Ig-like V-type domain at the N-terminus, followed by six Ig-like C2-type domains and a GPI anchor, and exists as a homodimer. CEACAM5 and CEACAM6 are overexpressed in many cancers and are associated with adhesion and invasion. CEACAM5 can mediate cell-cell adhesion through homotypic and heterotypic interactions. It functions as a homotypic intercellular adhesion molecule and serves as a widely used tumor marker, since it is expressed at higher levels in tumorous tissues than in corresponding normal tissues. CEACAM5 has also been shown to contribute to tumorigenicity by inhibiting cellular differentiation. In addition, CEACAM5 is identified as the host receptor for the Dr family of adhesins of E Coli, and the binding of E Coli Dr adhesins leads to dissociation of the CEACAM5 homodimer.

This CEACAM5 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human CEACAM5. It contains Human CEACAM5 capture antibody, Human CEACAM5 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human CEACAM5 protein.

Product Categories/Family for CEACAM5 elisa pair set

Cancer Drug Targets

References

Baczyska D, et al. (2003) The tumorigenic potential of human CX-1 colon adenocarcinoma cells depends on carcinoembryonic antigen (CEACAM5) expression. Cell Mol Biol Lett. 8(2): 471-86.
Blumenthal RD, et al. (2005) Inhibition of adhesion, invasion, and metastasis by antibodies targeting CEACAM6 (NCA-90) and CEACAM5 (Carcinoembryonic Antigen). Cancer Res. 65(19): 8809-17.
Liebig B, et al. (2005) Forced expression of deltaN-TCF-1B in colon cancer derived cell lines is accompanied by the induction of CEACAM5/6 and mesothelin. Cancer Lett. 223(1): 159-67.