Testing Data
CD160 elisa pair set
CD160 Matched ELISA Antibody Pair Set, Human
Synonyms
CD160; CD160 Matched ELISA Antibody Pair Set; Human; Human CD160 ELISA Pair Set; CD160 molecule; BY55 Matched ELISA Antibody Pair Set; NK1 Matched ELISA Antibody Pair Set; NK28 Matched ELISA Antibody Pair Set; CD160 elisa pair set
Specificity
Quantitative determination of Human CD160
Assay Type
Solid Phase Sandwich ELISA
Detection Range
93.75-6000pg/mL
Sensitivity
93.75pg/ml
Materials Provided
1. Capture Antibody 1.0 mg/mL of rabbit anti-CD160 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL rabbit anti-CD160 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.25ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 200 ng of recombinant CD160. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 6ng/mL is recommended.
2. Detection Antibody 0.2 mg/mL rabbit anti-CD160 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.25ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 200 ng of recombinant CD160. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 6ng/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for CD160 elisa pair set
CD16 antigen, also known as Natural killer cell receptor BY55 and CD16, is a cell membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. CD16 is a GPI-anchored lymphocyte surface receptor in which expression is mostly restricted to the highly cytotoxic CD56(dim)CD16(+) peripheral blood NK subset. CD16 is a receptor showing broad specificity for both classical and non-classical MHC class I molecules. CD16 is expressed in spleen, peripheral blood, and small intestine. Expression of CD16 is restricted to functional NK and T cytotoxic lymphocytes. CD16 acts as a co-activator receptor for CD3-induced proliferation of CD4+ CD16+ T cells isolated from inflammatory skin lesions. Unique CD4+ CD16+ lymphocyte subset may play a role in the pathogenesis of skin inflammation. Activated NK lymphocytes release a soluble form of CD16 that functionally impairs the MHC-I-specific cytotoxic CD8(+) T lymphocyte responsiveness.
This CD160 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human CD160. It contains Human CD160 capture antibody, Human CD160 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human CD160 protein.
This CD160 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human CD160. It contains Human CD160 capture antibody, Human CD160 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human CD160 protein.
Product Categories/Family for CD160 elisa pair set
References
Barakonyi A. et al., 2004, J Immunol.173 (9): 5349-54. Rabot M. et al., 2006, Transpl Immunol. 17 (1): 36-8. Abecassis S. et al., 2007, J Invest Dermatol. 127?(5): 1161-6. Giustiniani J.?et al., 2007, J Immunol. 178 (3): 1293-300.
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Product Notes
The CD160 (Catalog #AAA8118425) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CD160, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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