FOLR1 elisa pair set

FOLR1 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118367

• Synonyms: FOLR1; FOLR1 Matched ELISA Antibody Pair Set; Human; Human FOLR1 ELISA Pair Set; folate receptor 1 (adult); FBP Matched ELISA Antibody Pair Set; Folate Binding Protein Matched ELISA Antibody Pair Set; FOLR Matched ELISA Antibody Pair Set; FOLR1 elisa pair set

• Specificity: Quantitative determination of Human FOLR1

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 4.69-300pg/mL
• Sensitivity: 4.69pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of mouse anti-Human FOLR1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of mouse anti-Human FOLR1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.25ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 50 ng of recombinant Human FOLR1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 300 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for FOLR1 elisa pair set

The protein encoded by FOLR1 gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene.
Folate receptor alpha (FRalpha) is the most important subunit of Folate receptor and the alpha isoform has been shown to be selectively overexpressed in cancer types like breast and ovarian cancer compared to normal breast and ovarian epithelial cells. It was determined that Folate receptor alpha exhibits a limited expression on the apical surfaces of the epithelial cells of normal lung, breast, thyroid, parathyroid, and kidney tissues. For their uptake of folate, normal cells rely almost exclusively on the reduced folate carrier, whereas many carcinomas and myeloid leukemia cells overexpress a high-affinity FR on their surfaces, perhaps reflecting their increased need for folate to support rapid cell division.

This FOLR1 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human FOLR1. It contains Human FOLR1 capture antibody, Human FOLR1 detector antibody (HRP) and a highly purified recombinant Human FOLR1 protein.

References

Senol S, Ceyran AB, Aydin A, et al. Folate receptor alpha expression and significance in endometrioid endometrium carcinoma and endometrial hyperplasia. International Journal of Clinical and Experimental Pathology. 2015;8(5):5633-5641.