C-Reactive Protein ELISA Pair Set | CRP elisa pair set

C-Reactive Protein Matched ELISA Antibody Pair Set, Mouse

Cat. Num. AAA8118500

• Synonyms: C-Reactive Protein; C-Reactive Protein Matched ELISA Antibody Pair Set; Mouse; Mouse C-Reactive Protein/CRP ELISA Pair Set; C-reactive protein; pentraxin-related; AI255847 Matched ELISA Antibody Pair Set; CRP elisa pair set

• Specificity: Quantitative determination of Mouse C-Reactive Protein

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 34.38-2200pg/mL
• Sensitivity: 34.38pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of rabbit anti-Mouse C-Reactive Protein/CRP monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse C-Reactive Protein/CRP polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.25ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 110 ng of recombinant Mouse C-Reactive Protein/CRP. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2200 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for CRP elisa pair set

C-reactive protein (CRP) is synthesized by the liver in response to factors released by fat cells. It is a member of the pentraxin family of proteins. The levels of CRP rise in response to inflammation. Human C-reactive protein (CRP) is the classical acute phase reactant, the circulating concentration of which rises rapidly and extensively in a cytokine-mediated response to tissue injury, infection and inflammation. Serum CRP values are routinely measured, empirically, to detect and monitor many human diseases. However, CRP is likely to have important host defence, scavenging and metabolic functions through its capacity for calcium-dependent binding to exogenous and autologous molecules containing phosphocholine (PC) and then activating the classical complement pathway. CRP may also have pathogenic effects and the recent discovery of a prognostic association between increased CRP production and coronary atherothrombotic events is of particular interest.

This C-Reactive Protein Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse C-Reactive Protein. It contains Mouse C-Reactive Protein capture antibody, Mouse C-Reactive Protein detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse C-Reactive Protein protein.

References

Pepys MB. et al., 2003, J Clin Invest. 111 (12): 1805-12. Thompson D. et al., 1999, Structure. 7(2): 169-77.