Rat RANK ELISA Kit | RANK elisa kit

Rat RANK PicoKine Fast ELISA Kit

Cat. Num. AAA1752768

• Gene Names: Tnfrsf11a; RANK; RGD1563614
• Reactivity: Rat
• Synonyms: RANK; Rat RANK PicoKine Fast ELISA Kit; rat RANK; tumor necrosis factor receptor superfamily; member 11a; NFKB activator; Protein Tnfrsf11a; Tnfrsf11a; Tumor necrosis factor receptor superfamily member 11A; RANK elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat Tnfrsf11a

• Samples: Cell culture supernates and serum
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RANK elisa kit

Principle of the Assay: The Picokine™ Rat Tnfrsf11a Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Tnfrsf11a with a 96-well strip plate that is pre-coated with antibody specific for Tnfrsf11a. The detection antibody is a biotinylated antibody specific for Tnfrsf11a. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Tnfrsf11a with immunogen: Expression system for standard: NSO; Immunogen sequence: Q30-P213. The kit is analytically validated with ready to use reagents. To measure Rat Tnfrsf11a, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Rat Tnfrsf11a in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Tnfrsf11a in the sample.!!Background/Introduction: Receptor Activator of Nuclear Factor kappa B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain(residues 234-616), which contain three putative TRAF-binding domains(termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK. The standard in this kit is recombinant human RANK with the sequence of Q29-G213 aa. It is a dipolymer which compose of two chains, and the molecular weight of each is 48kda.

NCBI and Uniprot Product Information

• NCBI GeneID: 498206
• UniProt Accession #: F1M8Z6
• NCBI Official Synonym Full Names: TNF receptor superfamily member 11A
• NCBI Official Symbol: Tnfrsf11a
• NCBI Official Synonym Symbols: RANK; RGD1563614
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 11A
• UniProt Protein Name: Protein Tnfrsf11a
• UniProt Gene Name: Tnfrsf11a
• UniProt Entry Name: F1M8Z6_RAT