Mouse RANK ELISA Kit | TNFRSF11A elisa kit

Mouse RANK PicoKine ELISA Kit

Cat. Num. AAA175876

• Gene Names: Tnfrsf11a; OFE; ODFR; Rank; Ly109; mRANK; TRANCE-R
• Reactivity: Mouse
• Synonyms: RANK; Mouse RANK PicoKine ELISA Kit; Tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily; member 11a; NFKB activator; CD265; FEO; LOH18CR1; Loss of heterozygosity 18 chromosomal region 1; ODFR; OFE; OPTB7; Osteoclast differentiation factor receptor; OSTS; PDB2; Receptor activator of NF-KB; Receptor activator of nuclear factor kappa B; TNFRSF11A; TNR11_HUMAN; TRANCER; Tumor necrosis factor receptor superfamily member 11a activator of NFKB; Tumor necrosis factor receptor superfamily member 11a NFKB activator; TNFRSF11A elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Tnfrsf11a
• Sequence Length: 625

• Samples: Cell culture supernates and serum
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <2pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess interassay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNFRSF11A elisa kit

Principle of the Assay: The Picokine™ Mouse Tnfrsf11a Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Tnfrsf11a with a 96-well strip plate that is pre-coated with antibody specific for Tnfrsf11a. The detection antibody is a biotinylated antibody specific for Tnfrsf11a. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Tnfrsf11a with immunogen: Expression system for standard: NSO; Immunogen sequence: Q30-P213. The kit is analytically validated with ready to use reagents. To measure Mouse Tnfrsf11a, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Tnfrsf11a in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Tnfrsf11a in the sample.

Background/Introduction: Receptor Activator of Nuclear Factor kappa B(RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain(residues 234-616), which contain three putative TRAF-binding domains(termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK.

NCBI and Uniprot Product Information

• NCBI GI #: 17380323
• NCBI GeneID: 21934
• UniProt Accession #: O35305; Q8VCT7
• Molecular Weight: 66,621 Da
• NCBI Official Full Name: Tumor necrosis factor receptor superfamily member 11A
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 11a, NFKB activator
• NCBI Official Symbol: Tnfrsf11a
• NCBI Official Synonym Symbols: OFE; ODFR; Rank; Ly109; mRANK; TRANCE-R
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 11A; receptor activator of NF-KB; receptor activator of NF-kappaB; osteoclast differentiation factor receptor
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 11A
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: Tnfrsf11a
• UniProt Synonym Gene Names: Rank; ODFR
• UniProt Entry Name: TNR11_MOUSE

Uniprot Description

TNFRSF11A: Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. Binds to the clefts between the subunits of the TNFSF11 ligand trimer to form a heterohexamer. Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6. Interacts (via cytoplasmic domain) with GAB2. Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.

Protein type: Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; tumor necrosis factor receptor activity; transmembrane receptor activity; cytokine binding; metal ion binding

Biological Process: positive regulation of JNK activity; circadian thermoregulation; adaptive immune response; ossification; tumor necrosis factor-mediated signaling pathway; multicellular organismal development; response to cytokine stimulus; programmed cell death; response to lipopolysaccharide; positive regulation of transcription factor activity; osteoclast differentiation; lymph node development; activation of NF-kappaB transcription factor