Typical Testing Data/Standard Curve (for reference only)
STC2 elisa kit
Human STC2 PicoKine ELISA Kit
Gene Names
STC2; STC-2; STCRP
Reactivity
HumanThere is no detectable cross-reactivity with other relevant proteins.
Synonyms
STC2; Human STC2 PicoKine ELISA Kit; Human STC2; Stanniocalcin-2; STC-2; Stanniocalcin-related protein; STC-related protein; STCRP; Stanniocalcin 2; STC2 elisa kit
Reactivity
Human
There is no detectable cross-reactivity with other relevant proteins.
There is no detectable cross-reactivity with other relevant proteins.
Specificity
Natural and recombinant Human SMOC2
Sequence Length
302
Samples
Cell Culture Supernates, Serum And Plasma (Heparin, Edta)
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months.
Avoid multiple freeze-thaw cycles.
Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for STC2 elisa kit
Principle of the Assay: The Picokine Human SMOC2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human SMOC2 with a 96-well strip plate that is pre-coated with antibody specific for SMOC2. The detection antibody is a biotinylated antibody specific for SMOC2. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human SMOC2 with immunogen: Expression system for standard: CHO, Immunogen sequence: Q22-G446. The kit is analytically validated with ready to use reagents. To measure Human SMOC2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human SMOC2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human SMOC2 in the sample!!Background/Introduction: SPARC-related modular calcium-binding protein 2 is a protein that in humans is encoded by the SMOC2 gene. This gene encodes a member of the SPARC family (secreted protein acidic and rich in cysteine/osteonectin/BM-40), which are highly expressed during embryogenesis and wound healing. The gene product is a matricellular protein which promotes matrix assembly and can stimulate endothelial cell proliferation and migration, as well as angiogenic activity. Associated with pulmonary function, this secretory gene product contains a Kazal domain, two thymoglobulin type-1 domains, and two EF-hand calcium-binding domains. The encoded protein may serve as a target for controlling angiogenesis in tumor growth and myocardial ischemia. Alternative splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,249 Da
NCBI Official Full Name
stanniocalcin-2
NCBI Official Synonym Full Names
stanniocalcin 2
NCBI Official Symbol
STC2
NCBI Official Synonym Symbols
STC-2; STCRP
NCBI Protein Information
stanniocalcin-2
UniProt Protein Name
Stanniocalcin-2
Protein Family
UniProt Gene Name
STC2
UniProt Synonym Gene Names
STC-2; STC-related protein; STCRP
NCBI Description
This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers. [provided by RefSeq, Jul 2008]
Uniprot Description
Has an anti-hypocalcemic action on calcium and phosphate homeostasis.
Research Articles on STC2
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Product Notes
The Human There is no detectable cross-reactivity with other relevant proteins. STC2 stc2 (Catalog #AAA1751728) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1751728 ELISA Kit recognizes Human There is no detectable cross-reactivity with other relevant proteins. STC2. It is sometimes possible for the material contained within the vial of "STC2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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