Rat FGF23 ELISA Kit | FGF23 elisa kit

Rat FGF23 PicoKine ELISA Kit

Cat. Num. AAA1751556

• Gene Names: FGF23; ADHR; FGFN; HYPF; HFTC2; HPDR2; PHPTC
• Reactivity: Rat
• Synonyms: FGF23; Rat FGF23 PicoKine ELISA Kit; Rat FGF23; FGF23 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat Fgf21
• Sequence Length: 251

• Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta)
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF23 elisa kit

Principle of the Assay: The Picokine Rat Fgf21 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Fgf21 with a 96-well strip plate that is pre-coated with antibody specific for Fgf21. The detection antibody is a biotinylated antibody specific for Fgf21. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Rat Fgf21 with immunogen: Expression system for standard: E.coli, Immunogen sequence: Y30-S208. The kit is analytically validated with ready to use reagents. To measure Rat Fgf21, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Rat Fgf21 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Fgf21 in the sample!!Background/Introduction: Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al.(2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.

NCBI and Uniprot Product Information

• NCBI GI #: 10190674
• NCBI GeneID: 8074
• NCBI Accession #: NP_065689.1
• NCBI GenBank Nucleotide #: NM_020638.2
• NCBI Official Full Name: fibroblast growth factor 23
• NCBI Official Synonym Full Names: fibroblast growth factor 23
• NCBI Official Symbol: FGF23
• NCBI Official Synonym Symbols: ADHR; FGFN; HYPF; HFTC2; HPDR2; PHPTC
• NCBI Protein Information: fibroblast growth factor 23
• Protein Family: Fibroblast growth factor

NCBI Description

This gene encodes a member of the fibroblast growth factor family of proteins, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. The product of this gene regulates phosphate homeostasis and transport in the kidney. The full-length, functional protein may be deactivated via cleavage into N-terminal and C-terminal chains. Mutation of this cleavage site causes autosomal dominant hypophosphatemic rickets (ADHR). Mutations in this gene are also associated with hyperphosphatemic familial tumoral calcinosis (HFTC). [provided by RefSeq, Feb 2013]