Rat RANK ELISA Kit | TNFRSF11A elisa kit

Rat RANK PicoKine ELISA Kit

Cat. Num. AAA177203

• Gene Names: Tnfrsf11a; RANK; RGD1563614
• Reactivity: Rat
• Synonyms: RANK; Rat RANK PicoKine ELISA Kit; Tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily; member 11a; NFKB activator; CD265; FEO; LOH18CR1; Loss of heterozygosity 18 chromosomal region 1; ODFR; OFE; OPTB7; Osteoclast differentiation factor receptor; OSTS; PDB2; Receptor activator of NF-KB; Receptor activator of nuclear factor kappa B; TNFRSF11A; TNR11_HUMAN; TRANCER; Tumor necrosis factor receptor superfamily member 11a activator of NFKB; Tumor necrosis factor receptor superfamily member 11a NFKB activator; TNFRSF11A elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat RANK
• Sequence Length: 604

• Samples: Cell culture supernates and serum
• Assay Type: Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Rat RANK PicoKine ELISA Kit standard curve )

Related Product Information for TNFRSF11A elisa kit

Principle of the assay: rat RANK ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for RANK has been precoated onto 96-well plates. Standards(NSO, Q30-P213) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for RANK is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat RANK amount of sample captured in plate.
Background: Receptor Activator of Nuclear Factor kappa B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain(residues 234-616), which contain three putative TRAF-binding domains(termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK. The standard in this kit is recombinant human RANK with the sequence of Q29-G213 aa. It is a dipolymer which compose of two chains, and the molecular weight of each is 48kda.

NCBI and Uniprot Product Information

• NCBI GeneID: 498206
• UniProt Accession #: F1M8Z6
• Molecular Weight: 64,413 Da
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 11a, NFKB activator
• NCBI Official Symbol: Tnfrsf11a
• NCBI Official Synonym Symbols: RANK; RGD1563614
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 11A
• UniProt Protein Name: Protein Tnfrsf11a
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: Tnfrsf11a
• UniProt Entry Name: F1M8Z6_RAT