Rabbit anti-Human SPT1 Polyclonal Antibody | anti-SPTLC1 antibody

SPT1 Antibody

Cat. Num. AAA150884

• Gene Names: SPTLC1; HSN1; LBC1; LCB1; SPT1; SPTI; HSAN1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: SPT1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: SPT1; Polyclonal Antibody; SPT1 Antibody; HSN1; LBC1; LCB1; SPTI; HSAN1; Serine palmitoyltransferase 1; Long chain base biosynthesis protein 1; LCB 1; serine palmitoyltransferase; long chain base subunit 1; anti-SPTLC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least two isoforms of SPT1 are known to exist; this antibody will detect only isoform a. SPT1 antibody is predicted to not cross-react with SPT2.
• Purity/Purification: SPT1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 473

• Applicable Applications for anti-SPTLC1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: SPT1 antibody can be used for detection of SPT1 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: SPT1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SPT1.
• Buffer: SPT1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: SPT1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of SPT1 in (A) A549 and (B) HeLa cell lysate with SPT1 antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of SPT1 in human lung tissue with SPT1 antibody at 5 μg/mL.)

Related Product Information for anti-SPTLC1 antibody

SPT1 Antibody: Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPT1 is the long chain base subunit 1 of mammalian serine palmitoyltransferase. SPT1 is not catalytically active but is necessary for the stabilization of the SPT2 subunit and anchoring the holoenzyme to the cytosolic face of the endoplasmic reticulum. Missense mutations in this gene have been identified in patients with hereditary sensory neuropathy type 1 (HSAN1). These mutations induce a shift in the substrate specificity of the holoenzyme, leading to the formation and accumulation of two neurotoxic sphingolipids.

NCBI and Uniprot Product Information

• NCBI GI #: 5454084
• NCBI GeneID: 10558
• NCBI Accession #: NP_6406
• NCBI GenBank Nucleotide #: NM_006415.3
• UniProt Accession #: O15269; Q5VWB4; Q96IX6; A8K681
• Molecular Weight: 16,073 Da
• NCBI Official Full Name: serine palmitoyltransferase 1 isoform a
• NCBI Official Synonym Full Names: serine palmitoyltransferase, long chain base subunit 1
• NCBI Official Symbol: SPTLC1
• NCBI Official Synonym Symbols: HSN1; LBC1; LCB1; SPT1; SPTI; HSAN1
• NCBI Protein Information: serine palmitoyltransferase 1; LCB 1; SPT 1; serine C-palmitoyltransferase; serine-palmitoyl-CoA transferase 1; long chain base biosynthesis protein 1
• UniProt Protein Name: Serine palmitoyltransferase 1
• Protein Family: Serine palmitoyltransferase
• UniProt Gene Name: SPTLC1
• UniProt Synonym Gene Names: LCB1; LCB 1; SPT 1; SPT1
• UniProt Entry Name: SPTC1_HUMAN

NCBI Description

This gene encodes a member of the class-II pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is the long chain base subunit 1 of serine palmitoyltransferase. Serine palmitoyltransferase converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate and is the key enzyme in sphingolipid biosynthesis. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified. Pseudogenes of this gene have been defined on chromosomes 1, 6, 10, and 13. [provided by RefSeq, Jul 2013]

Uniprot Description

SPTLC1: Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1- SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Defects in SPTLC1 are the cause of hereditary sensory and autonomic neuropathy type 1A (HSAN1A). The hereditary sensory and autonomic neuropathies are a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN1A is an autosomal dominant axonal neuropathy with onset in the second or third decades. Initial symptoms are loss of pain, touch, heat, and cold sensation over the feet, followed by distal muscle wasting and weakness. Loss of pain sensation leads to chronic skin ulcers and distal amputations. Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 2.3.1.50; Transferase; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 9q22.2

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: serine C-palmitoyltransferase activity; protein binding; pyridoxal phosphate binding

Biological Process: sphingolipid metabolic process; sphingolipid biosynthetic process; sphingosine biosynthetic process; ceramide biosynthetic process; sphinganine biosynthetic process; sphingomyelin biosynthetic process

Disease: Neuropathy, Hereditary Sensory And Autonomic, Type Ia