Human CDCP1 ELISA Kit | CDCP1 elisa kit

Human CDCP1 PicoKine ELISA Kit

Cat. Num. AAA1751585

• Gene Names: CDCP1; CD318; TRASK; SIMA135
• Reactivity: Human
• Synonyms: CDCP1; Human CDCP1 PicoKine ELISA Kit; Human CDCP1; CDCP1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CD80
• Sequence Length: 836

• Samples: Cell Culture Supernates And Serum
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDCP1 elisa kit

Principle of the Assay: The Picokine Human CD80 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CD80 with a 96-well strip plate that is pre-coated with antibody specific for CD80. The detection antibody is a biotinylated antibody specific for CD80. The capture antibody is monoclonal antibody from mouse, the detection antibody is monoclonal antibody from mouse. The kit contains recombinant Human CD80 with immunogen: Expression system for standard: NSO; Immunogen sequence: V35-N242. The kit is analytically validated with ready to use reagents. To measure Human CD80, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human CD80 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD80 in the sample!!Background/Introduction: The protein CD80(Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

NCBI and Uniprot Product Information

• NCBI GI #: 30410805
• NCBI GeneID: 64866
• NCBI Accession #: NP_073753.3
• NCBI GenBank Nucleotide #: NM_022842.5
• UniProt Accession #: Q9H5V8
• NCBI Official Full Name: CUB domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: CUB domain containing protein 1
• NCBI Official Symbol: CDCP1
• NCBI Official Synonym Symbols: CD318; TRASK; SIMA135
• NCBI Protein Information: CUB domain-containing protein 1
• Protein Family: CUB domain-containing protein

NCBI Description

This gene encodes a transmembrane protein which contains three extracellular CUB domains and acts as a substrate for Src family kinases. The protein plays a role in the tyrosine phosphorylation-dependent regulation of cellular events that are involved in tumor invasion and metastasis. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]