Rat CES3 ELISA Kit | CES3 elisa kit

Rat CES3 PicoKine ELISA Kit

Cat. Num. AAA1751642

• Gene Names: CES3; ES31
• Reactivity: Rat
• Synonyms: CES3; Rat CES3 PicoKine ELISA Kit; Rat CES3; CES3 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat Cdh3
• Sequence Length: 571

• Samples: Cell Culture Supernates And Serum
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <5pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CES3 elisa kit

Principle of the Assay: The Picokine Rat Cdh3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Cdh3 with a 96-well strip plate that is pre-coated with antibody specific for Cdh3. The detection antibody is a biotinylated antibody specific for Cdh3. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Rat Cdh3 with immunogen: Expression system for standard: NSO, Immunogen sequence: E100-G647. The kit is analytically validated with ready to use reagents. To measure Rat Cdh3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Rat Cdh3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Cdh3 in the sample!!Background/Introduction: Cadherins are calcium-dependent cell-cell adhesion molecules that mediate cell-cell binding in a homophilic manner. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherin expression is regulated spatially as well as temporally. Cadherins are thought to play an important role in development and maintenance of tissues through selective cell-cell adhesion activity and may be involved also in the invasion and metastasis of malignant tumors. Cadherin regulates dendritic spine morphogenesis. A cadherin gene cluster is mapped to a region of chromosome 5 subject to frequent allelic loss in carcinoma. The standard product used in this kit is recombinant P-Cadherin with the molecular mass of 120-130Kda after glycosylation.

NCBI and Uniprot Product Information

• NCBI GI #: 33563374
• NCBI GeneID: 23491
• NCBI Accession #: NP_079198.2
• NCBI GenBank Nucleotide #: NM_024922.6
• UniProt Accession #: Q6UWW8
• NCBI Official Full Name: carboxylesterase 3 isoform 1
• NCBI Official Synonym Full Names: carboxylesterase 3
• NCBI Official Symbol: CES3
• NCBI Official Synonym Symbols: ES31
• NCBI Protein Information: carboxylesterase 3
• Protein Family: Carboxylesterase

NCBI Description

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This gene is expressed in several tissues, particularly in colon, trachea and in brain, and the protein participates in colon and neural drug metabolism. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported, but the biological validity and/or full-length nature of some variants have not been determined.[provided by RefSeq, Jun 2010]