Typical Testing Data/Standard Curve (for reference only)
Rat Serpin A12 ELISA Kit | SERPINA12 elisa kit
Rat Serpin A12 PicoKine ELISA Kit
Reactivity
Rat
Synonyms
Serpin A12; Rat Serpin A12 PicoKine ELISA Kit; Rat Serpin A12; serpin family A member 12; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin; Serpina12; SERPINA12 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant Rat Serpina12
Sequence Length
411
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SERPINA12 elisa kit
Principle of the Assay: The Picokine™ Rat Serpina12 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Serpina12 with a 96-well strip plate that is pre-coated with antibody specific for Serpina12. The detection antibody is a biotinylated antibody specific for Serpina12. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Rat Serpina12 with immunogen: Expression system for standard: NSO, Immunogen sequence: L21-P411. The kit is analytically validated with ready to use reagents. To measure Rat Serpina12, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat Serpina12 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Serpina12 in the sample.
NCBI and Uniprot Product Information
Uniprot Description
Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.
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Product Notes
The Rat SERPINA12 serpina12 (Catalog #AAA1752561) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1752561 ELISA Kit recognizes Rat SERPINA12. It is sometimes possible for the material contained within the vial of "Serpin A12, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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