Typical Testing Data/Standard Curve (for reference only)
Rat IL6ST ELISA Kit | IL6ST elisa kit
Rat IL6ST PicoKine ELISA Kit
Gene Names
IL6ST; CD130; GP130; CDW130; IL-6RB
Reactivity
Rat
Synonyms
IL6ST; Rat IL6ST PicoKine ELISA Kit; Rat IL6ST; IL6ST elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat IL6ST
Sequence Length
918
Samples
Cell Culture Supernates, Cell Lysates, Serum And Plasma (Heparin, Edta)
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL6ST elisa kit
Principle of the Assay: The Picokine Rat Il1b Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Il1b with a 96-well strip plate that is pre-coated with antibody specific for Il1b. The detection antibody is a biotinylated antibody specific for Il1b. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Il1b with immunogen: Expression system for standard: E.coli,V117-S268. The kit is analytically validated with ready to use reagents. To measure Rat Il1b, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Rat Il1b in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Il1b in the sample!!Background/Introduction: The Interleukin 1 family is a group of 11 beta trefoil cytokines. Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta belongs to proinflammatory cytokines, promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1(VCAM-1) on hepatic sinusoidal endothelium(HSE).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
interleukin-6 receptor subunit beta isoform 1
NCBI Official Synonym Full Names
interleukin 6 signal transducer
NCBI Official Symbol
IL6ST
NCBI Official Synonym Symbols
CD130; GP130; CDW130; IL-6RB
NCBI Protein Information
interleukin-6 receptor subunit beta
Protein Family
NCBI Description
The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014]
Research Articles on IL6ST
Similar Products
Product Notes
The Rat IL6ST (Catalog #AAA1751622) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1751622 ELISA Kit recognizes Rat IL6ST. It is sometimes possible for the material contained within the vial of "IL6ST, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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