Human JAM-C ELISA Kit | JAM-C elisa kit

Human JAM-C PicoKine ELISA Kit

Cat. Num. AAA1751656

• Gene Names: JAM3; JAMC; JAM-2; JAM-3; JAM-C
• Reactivity: Human
• Synonyms: JAM-C; Human JAM-C PicoKine ELISA Kit; Human JAM-C; JAM-C elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human JAM2
• Sequence Length: 259

• Samples: Cell Culture Supernates, Serum And Plasma(Heparin, Edta)
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for JAM-C elisa kit

Principle of the Assay: The Picokine Human JAM2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human JAM2 with a 96-well strip plate that is pre-coated with antibody specific for JAM2. The detection antibody is a biotinylated antibody specific for JAM2. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human JAM2 with immunogen: Expression system for standard: NSO, Immunogen sequence: F29-S238. The kit is analytically validated with ready to use reagents.To measure Human JAM2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human JAM2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human JAM2 in the sample!!Background/Introduction: Junctional adhesion molecule B is a protein that in humans is encoded by the JAM2 gene. It is mapped to 21q21.3. This gene belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. The protein encoded by this gene is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs. Alternatively spliced transcript variants have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 328927057
• NCBI GeneID: 83700
• NCBI Accession #: NP_001192258.1
• NCBI GenBank Nucleotide #: NM_001205329.1
• NCBI Official Full Name: junctional adhesion molecule C isoform 2
• NCBI Official Synonym Full Names: junctional adhesion molecule 3
• NCBI Official Symbol: JAM3
• NCBI Official Synonym Symbols: JAMC; JAM-2; JAM-3; JAM-C
• NCBI Protein Information: junctional adhesion molecule C

NCBI Description

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. A mutation in an intron of this gene is associated with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]