Mouse IL-2 ELISA Kit | IL-2 elisa kit

Mouse IL-2 PicoKine Fast ELISA Kit

Cat. Num. AAA1752718

• Gene Names: Il2; Il-2
• Reactivity: Mouse
• Synonyms: IL-2; Mouse IL-2 PicoKine Fast ELISA Kit; Mouse IL-2; Interleukin-2; T-cell growth factor; TCGF; Il2; Il-2; IL-2 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Il2
• Sequence Length: 169

• Samples: Cell culture supernates, serum and plasma(heparin, EDTA, citrate)
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <1pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-2 elisa kit

Principle of the Assay: The Picokine™ Mouse Il2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Il2 with a 96-well strip plate that is pre-coated with antibody specific for Il2. The detection antibody is a biotinylated antibody specific for Il2. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Il2 with immunogen: Expression system for standard: E.coli,A21-Q169. The kit is analytically validated with ready to use reagents. To measure Mouse Il2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse Il2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Il2 in the sample.!!Background/Introduction: Interleukin-2(IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigenactivated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2(IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2(IL2) is assigned to chromosome 4. Mouse IL-2 contains 149 amino acids in mature form with the molecular mass of 17.2KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 7110653
• NCBI GeneID: 16183
• NCBI Accession #: NP_032392.1
• NCBI GenBank Nucleotide #: NP_032392.1
• UniProt Accession #: P04351
• NCBI Official Full Name: interleukin-2
• NCBI Official Synonym Full Names: interleukin 2
• NCBI Official Symbol: Il2
• NCBI Official Synonym Symbols: Il-2
• NCBI Protein Information: interleukin-2
• UniProt Protein Name: Interleukin-2
• UniProt Gene Name: Il2
• UniProt Synonym Gene Names: Il-2; IL-2; TCGF
• UniProt Entry Name: IL2_MOUSE

Uniprot Description

IL2: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. Belongs to the IL-2 family.

Protein type: Secreted, signal peptide; Secreted; Oncoprotein; Cytokine

Cellular Component: extracellular space; cell; extracellular region; cytosol

Molecular Function: growth factor activity; interleukin-2 receptor binding; cytokine activity; carbohydrate binding; glycosphingolipid binding; kappa-type opioid receptor binding

Biological Process: positive regulation of interleukin-17 production; positive regulation of isotype switching to IgG isotypes; regulation of T cell homeostatic proliferation; positive regulation of regulatory T cell differentiation; negative regulation of heart contraction; immune system process; positive regulation of immunoglobulin secretion; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; positive regulation of interferon-gamma production; negative regulation of protein amino acid phosphorylation; positive regulation of T cell differentiation; positive regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of inflammatory response; protein kinase C activation; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of protein amino acid phosphorylation; negative regulation of B cell apoptosis