Rabbit anti-Human CXADR Polyclonal Antibody | anti-CXADR antibody

CXADR, ID (CXADR, CAR, Coxsackievirus and adenovirus receptor, CVB3-binding protein, Coxsackievirus B-adenovirus receptor, HCVADR) (MaxLight 550)

Cat. Num. AAA6290184

• Gene Names: CXADR; CAR; HCAR; CAR4/6
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: CXADR; Polyclonal Antibody; ID (CXADR; CAR; Coxsackievirus and adenovirus receptor; CVB3-binding protein; Coxsackievirus B-adenovirus receptor; HCVADR) (MaxLight 550); HCVADR; anti-CXADR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-CXADR antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: CXADR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140~169 amino acids from the Center region of human CXADR.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CXADR Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). CXADR(arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NCI-H460 cells using CXADR Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CXADR antibody

CXADR is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses.

Product Categories/Family for anti-CXADR antibody

Antibodies; Infectious Disease Adenovirus

References

Tomko R.P., Xu R., Philipson L.Proc. Natl. Acad. Sci. U.S.A. 94:3352-3356(1997). Bowles K.R., Gibson J., Hum. Genet. 105:354-359(1999). Fechner H., Haack A., Wang H., Wang X.Gene Ther. 6:1520-1535(1999). Martino T.A., Petric M., Weingartl H.Virology 271:99-108(2000). Ashbourne-Excoffon K.J.D., Hruska-Hageman A.M.J. Cell Sci. 117:4401-4409(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 333470703
• NCBI GeneID: 1525
• NCBI Accession #: NP_001193992.1
• NCBI GenBank Nucleotide #: NM_001207063.1
• UniProt Accession #: P78310; O00694; Q8WWT6; Q8WWT7; Q8WWT8; Q9UKV4; B2R8V8
• Molecular Weight: 40,030 Da
• NCBI Official Full Name: coxsackievirus and adenovirus receptor isoform 2
• NCBI Official Synonym Full Names: coxsackie virus and adenovirus receptor
• NCBI Official Symbol: CXADR
• NCBI Official Synonym Symbols: CAR; HCAR; CAR4/6
• NCBI Protein Information: coxsackievirus and adenovirus receptor; HCVADR; CVB3-binding protein; coxsackievirus B-adenovirus receptor; 46 kD coxsackievirus and adenovirus receptor (CAR) protein
• UniProt Protein Name: Coxsackievirus and adenovirus receptor
• Protein Family: CXADR-like membrane protein
• UniProt Gene Name: CXADR
• UniProt Synonym Gene Names: CAR; CAR; hCAR
• UniProt Entry Name: CXAR_HUMAN

NCBI Description

The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq, May 2011]

Uniprot Description

CXADR: a component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1 located in the plasma membrane of PMN.

Protein type: Membrane protein, integral; Receptor, misc.; Cell adhesion

Chromosomal Location of Human Ortholog: 21q21.1

Cellular Component: extracellular space; neuron projection; protein complex; tight junction; integral to plasma membrane; basolateral plasma membrane; apicolateral plasma membrane; acrosome; extracellular region; intercellular junction; lipid raft; nucleoplasm; adherens junction; growth cone; plasma membrane; neuromuscular junction; cell junction; filopodium

Molecular Function: viral receptor activity; integrin binding; identical protein binding; protein binding; beta-catenin binding; cell adhesion molecule binding; PDZ domain binding; receptor binding

Biological Process: mitochondrion organization and biogenesis; neutrophil chemotaxis; intercellular junction assembly and maintenance; entry of virus into host cell; regulation of immune response; heart development; gamma-delta T cell activation; heterophilic cell adhesion; cardiac muscle fiber development; actin cytoskeleton reorganization; germ cell migration; homotypic cell-cell adhesion; blood coagulation; defense response to virus; leukocyte migration