Typical Testing Data/Standard Curve (for reference only)
Rat Galectin-1 ELISA Kit | LGALS1 elisa kit
Rat Galectin-1 PicoKine ELISA Kit
Gene Names
LGALS1; GBP; GAL1
Reactivity
Rat
Synonyms
Galectin-1; Rat Galectin-1 PicoKine ELISA Kit; Rat Galectin-1; LGALS1 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant Rat Jam3
Sequence Length
135
Samples
Cell Culture Supernates, Serum And Plasma(Heparin, Edta)
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
<5pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LGALS1 elisa kit
Principle of the Assay: The Picokine Rat Jam3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Jam3 with a 96-well strip plate that is pre-coated with antibody specific for Jam3. The detection antibody is a biotinylated antibody specific for Jam3. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Rat Jam3 with immunogen: Expression system for standard: NSO, Immunogen sequence: V32-N241. The kit is analytically validated with ready to use reagents. To measure Rat Jam3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Rat Jam3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Jam3 in the sample!!Background/Introduction: JAM3 belongs to the junctional adhesion molecule protein family and functions as a receptor for another member of this family. This gene is mapped to 8q13. JAM3 plays a part in cell-cell adhesion. The soluble form of JAM3 is a mediator of angiogenesis. JAM3 is a counter-receptor for ITGAM, mediating leukocyteplatelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). A mutation in an intron of the JAM3 gene is linked with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
galectin-1
NCBI Official Synonym Full Names
galectin 1
NCBI Official Symbol
LGALS1
NCBI Official Synonym Symbols
GBP; GAL1
NCBI Protein Information
galectin-1
Protein Family
NCBI Description
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008]
Research Articles on LGALS1
Similar Products
Product Notes
The Rat LGALS1 (Catalog #AAA1751554) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1751554 ELISA Kit recognizes Rat LGALS1. It is sometimes possible for the material contained within the vial of "Galectin-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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