Mouse OPG (TNFRSF11B)/Osteoprotegerin ELISA Kit | OPG elisa kit

Mouse OPG (TNFRSF11B)/Osteoprotegerin PicoKine Fast ELISA Kit

Cat. Num. AAA1752724

• Gene Names: Tnfrsf11b; Opg; TR1; OCIF
• Reactivity: Mouse
• Synonyms: OPG (TNFRSF11B)/Osteoprotegerin; Mouse OPG (TNFRSF11B)/Osteoprotegerin PicoKine Fast ELISA Kit; Mouse OPG; Tumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; Tnfrsf11b; Ocif; Opg; OPG elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Tnfrsf11b
• Sequence Length: 401

• Samples: Cell culture supernates, serum and plasma(EDTA)
• Detection Range: 93.7pg/ml-6000pg/ml
• Sensitivity: <5pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OPG elisa kit

Principle of the Assay: The Picokine™ Mouse Tnfrsf11b Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Tnfrsf11b with a 96-well strip plate that is pre-coated with antibody specific for Tnfrsf11b. The detection antibody is a biotinylated antibody specific for Tnfrsf11b. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Tnfrsf11b with immunogen: Expression system for standard: NSO; Immunogen sequence: E22-L401. The kit is analytically validated with ready to use reagents. To measure Mouse Tnfrsf11b, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse Tnfrsf11b in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Tnfrsf11b in the sample.!!Background/Introduction: Osteoprotegerin(OPG) is identical to osteoclastogenesis inhibitory factor(OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system.

NCBI and Uniprot Product Information

• NCBI GI #: 113930716
• NCBI GeneID: 18383
• NCBI Accession #: NP_032790.3
• NCBI GenBank Nucleotide #: NP_032790.3
• UniProt Accession #: O08712
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 11B
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
• NCBI Official Symbol: Tnfrsf11b
• NCBI Official Synonym Symbols: Opg; TR1; OCIF
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 11B
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 11B
• UniProt Gene Name: Tnfrsf11b
• UniProt Synonym Gene Names: Ocif; Opg

Uniprot Description

TNFRSF11B: Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis. Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD); also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast- mediated skeletal resorption, the disease can be fatal.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15|15 D1

Cellular Component: extracellular space; integral to plasma membrane; proteinaceous extracellular matrix

Molecular Function: tumor necrosis factor receptor activity

Biological Process: extracellular matrix organization and biogenesis; immune response; inflammatory response; negative regulation of bone resorption; negative regulation of odontogenesis of dentine-containing teeth; positive regulation of MAPKKK cascade; regulation of apoptosis; regulation of cell proliferation; response to lipopolysaccharide