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Western Blot (WB)
(Anti-YIF1A rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:32 kDa)
Rabbit anti-Human YIF1A Polyclonal Antibody | anti-YIF1A antibody
Anti-YIF1A Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
YIF1A; Polyclonal Antibody; Anti-YIF1A Antibody; Rabbit Polyclonal; YIF1A Antibody; Rabbit PAb; Antigen Affinity Purified; Yip1 interacting factor homolog A (S. cerevisiae); Anti-54TM Antibody; Anti-FinGER7 Antibody; Anti-YIF1 Antibody; Anti-YIF1P Antibody; anti-YIF1A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human YIF1A
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-YIF1A antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IHC-P: 1:250-1:1000
IP: 0.2-1uL/mg of lysate
IHC-P: 1:250-1:1000
IP: 0.2-1uL/mg of lysate
Immunogen
E Coli-derived Human YIF1A fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human YIF1A fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-YIF1A rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:32 kDa)
Western Blot (WB)
(Anti-YIF1A rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:32 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human YIF1A in human gallbladder with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human YIF1A in human gallbladder with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human YIF1A in human breast with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human YIF1A in human breast with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunoprecipitation (IP)
(YIF1A was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 1 uL anti-YIF1A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-YIF1A rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa Observed band size :22 kDa)
Immunoprecipitation (IP)
(YIF1A was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 1 uL anti-YIF1A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-YIF1A rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa Observed band size :22 kDa)
Related Product Information for anti-YIF1A antibody
YIF1A (Yip1 Interacting Factor Homolog A, Membrane Trafficking Protein) is a Protein Coding gene. The YIF1A gene, located on 11q13.2, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, zebrafish, C.elegans, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, and rice. YIF1A belongs to the YIF1 family and plays a possible role in transport between the endoplasmic reticulum and Golgi. YIF1A is widely expressed in the duodenum, liver, and other tissues. It is involved in the maintenance of the Golgi structure. Diseases associated with YIF1A include Amyotrophic Lateral Sclerosis 8 and Finger Agnosia. Missorting of YIF1A may contribute to VAPB-associated motor neuron disease.
References
Yoshida Y, et al. (2008) Yipf5 and yif1a recycle between the er and the golgi apparatus and are involved in the maintenance of the golgi structure. Exp Cell Res 314 (19): 3427-3443.Vitale G, et al. (1995) The gdp/gtp cycle of rab5 in the regulation of endocytotic membrane traffic. Cold Spring Harb Symp Quant Biol 60 211-220.Jin C, et al. (2005) Human yip1a specifies the localization of yif1 to the golgi apparatus. Biochem Biophys Res Commun 334 (1): 16-22.
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Product Notes
The YIF1A (Catalog #AAA8108995) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-YIF1A Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's YIF1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:1000 IHC-P: 1:250-1:1000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the YIF1A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YIF1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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