Western Blot (WB)
(Anti-PITX3 rabbit polyclonal antibody at 1:500 dilution Lane A: U-87 MG Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:32 kDa)
Rabbit PITX3/PTX3 Polyclonal Antibody | anti-PITX3 antibody
Anti-PITX3/PTX3 Antibody, Rabbit Polyclonal
Reactivity
Human, Cynomolgus
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
PITX3/PTX3; Polyclonal Antibody; Anti-PITX3/PTX3 Antibody; Rabbit Polyclonal; PITX3 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-ASGD1 Antibody; Anti-ASMD Antibody; Anti-ASOD Antibody; Anti-CTPP4 Antibody; Anti-CTRCT11 Antibody; Anti-PTX3 Antibody; anti-PITX3 antibody
Host
Rabbit
Reactivity
Human, Cynomolgus
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PITX3/PTX3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PITX3 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IHC-P: 1:250-1:1000
IP: 0.2-1uL/mg of lysate
IHC-P: 1:250-1:1000
IP: 0.2-1uL/mg of lysate
Immunogen
E Coli-derived Human PITX3 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PITX3 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PITX3 rabbit polyclonal antibody at 1:500 dilution Lane A: U-87 MG Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:32 kDa)
Western Blot (WB)
(Anti-PITX3 rabbit polyclonal antibody at 1:500 dilution Lane A: U-87 MG Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:32 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human PITX3 in cynomolgus cerebellum with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PITX3 in cynomolgus cerebellum with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunoprecipitation (IP)
(PITX3 was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate 1 uL anti-PITX3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PITX3 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa Observed band size :32 kDa)
Immunoprecipitation (IP)
(PITX3 was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate 1 uL anti-PITX3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PITX3 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa Observed band size :32 kDa)
Related Product Information for anti-PITX3 antibody
Pituitary homeobox 3 (Pitx3) is a bicoid-related homeodomain transcription factor that plays an important role in the development and maintenance of midbrain dopaminergic (mDA) neurons, ocular lens and skeletal muscle. In humans, mutations in PITX3 are responsible for cataracts and anterior segment abnormalities of varying degree; polymorphisms are associated with Parkinson's disease. In aphakia (ak) mice, two deletions in the promoter region of Pitx3 cause abnormal lens development.
References
Zazo Seco C, et al. (2018) Identification of pitx3 mutations in individuals with various ocular developmental defects. Ophthalmic Genet 39 (3): 314-320.Park S, et al. (2019) Establishment of pitx3-mcherry knock-in reporter human embryonic stem cell line (wae009-a-23). Stem Cell Res 39 101499.Chen J, et al. (2017) Impact of pitx3 gene knockdown on glial cell line-derived neurotrophic factor transcriptional activity in dopaminergic neurons. Neural Regen Res 12 (8): 1347-1351.
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Product Notes
The PITX3 (Catalog #AAA8109635) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PITX3/PTX3 Antibody, Rabbit Polyclonal reacts with Human, Cynomolgus and may cross-react with other species as described in the data sheet. AAA Biotech's PITX3/PTX3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:1000 IHC-P: 1:250-1:1000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the PITX3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PITX3/PTX3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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