Rabbit anti-Human ASB2 Polyclonal Antibody | anti-ASB2 antibody

ASB2, NT (ASB2, Ankyrin repeat and SOCS box protein 2) (Biotin)

Cat. Num. AAA6275536

• Gene Names: ASB2; ASB-2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ASB2; Polyclonal Antibody; NT (ASB2; Ankyrin repeat and SOCS box protein 2) (Biotin); Ankyrin repeat and SOCS box protein 2; anti-ASB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 587

• Applicable Applications for anti-ASB2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ASB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-86 amino acids from the N-terminal region of human ASB2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ASB2 Antibody (N-term) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the ASB2 antibody detected the ASB2 protein (arrow).)

Related Product Information for anti-ASB2 antibody

The protein encoded by this gene is a member of the ankyrin repeat and SOCS box-containing (ASB) family of proteins. They contain ankyrin repeat sequence and SOCS box domain. The SOCS box serves to couple suppressor of cytokine signalling (SOCS) proteins and their binding partners with the elongin B and C complex, possibly targeting them for degradation. This gene is induced by all-trans retinoic acid. In myeloid leukemia cells, the expression of this encoded protein has been shown to induce growth inhibition and chromatin condensation. Multiple alternatively spliced transcript variants have been described for this gene but their full length sequences are not known.

Product Categories/Family for anti-ASB2 antibody

Antibodies; SOCS

References

Heuze, M.L., et al. Blood 112(13):5130-5140(2008). Kohroki, J., et al. FEBS Lett. 579(30):6796-6802(2005). Heuze, M.L., et al. J. Biol. Chem. 280(7):5468-5474(2005). Colland, F., et al. Genome Res. 14(7):1324-1332(2004). Namciu, S.J., et al. Mamm. Genome 15(3):162-178(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 18252778
• NCBI GeneID: 51676
• NCBI Accession #: NP_057234.2
• NCBI GenBank Nucleotide #: NP_057234.2
• UniProt Accession #: Q96Q27
• NCBI Official Full Name: ankyrin repeat and SOCS box protein 2 isoform 2
• NCBI Official Synonym Full Names: ankyrin repeat and SOCS box containing 2
• NCBI Official Symbol: ASB2
• NCBI Official Synonym Symbols: ASB-2
• NCBI Protein Information: ankyrin repeat and SOCS box protein 2
• UniProt Protein Name: Ankyrin repeat and SOCS box protein 2
• Protein Family: Anthranilate synthase
• UniProt Gene Name: ASB2
• UniProt Synonym Gene Names: ASB-2
• UniProt Entry Name: ASB2_HUMAN

NCBI Description

This gene encodes a member of the ankyrin repeat and SOCS box-containing (ASB) protein family. These proteins play a role in protein degradation by coupling suppressor of cytokine signalling (SOCS) proteins with the elongin BC complex. The encoded protein is a subunit of a multimeric E3 ubiquitin ligase complex that mediates the degradation of actin-binding proteins. This gene plays a role in retinoic acid-induced growth inhibition and differentiation of myeloid leukemia cells. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]