Human SAP/PTX2 ELISA Kit | APCS elisa kit

Human SAP/PTX2 PicoKine ELISA Kit

Cat. Num. AAA178745

• Gene Names: APCS; SAP; PTX2; HEL-S-92n
• Reactivity: Human
• Synonyms: SAP/PTX2; Human SAP/PTX2 PicoKine ELISA Kit; 9.5S alpha 1 glycoprotein; 9.5S alpha-1-glycoprotein; APCS; P02743; PTX2; Ptx2; Sap; SAMP; SAP; Serum Amyloid P; Serum amyloid Pcomponent; Serum amyloid P-component; Serum amyloid P-component(1-203); APCS elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human SAP
• Sequence Length: 223

• Assay Range: 1.56ng/ml-100ng/ml
• Sensitivity: <10pg/ml
• Anticoagulant: heparin or EDTA
• TMB incubation time: 25-30
• Immunogen: E Coli, H20-V223
• Sample Data: Concentration(ng/ml): |0|1.56|3.12|6.25|12.5|25|50|100; O.D.: |0.049|0.127|0.204|0.378|0.657|1.038|1.587|1.963
• Intra/Inter Assay Precision: Sample: |1|2|3|1|2|3; n: |16|16|16|24|24|24; Mean(ng/ml): |2.14|8.51|33.18|2.88|10.26|42.16; Standard Deviation: |0.111|0.442|1.957|0.204|0.707|2.529; CV(%): |5.2|5.2|5.9|7.1|6.9|6.0
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 100ng/ml, 50ng/ml, 25ng/ml, 12.5ng/ml, 6.25ng/ml, 3.12ng/ml, 1.56ng/ml human SAP standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. We recommend that each human SAP standard solution and each sample is measured in duplicate.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human SAP/PTX2 PicoKine ELISA Kit standard curve )

Related Product Information for APCS elisa kit

For quantitative detection of human SAP in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: Amyloid P component, serum (SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.
Principle: Human SAP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for SAP has been precoated onto 96-well plates. Standards (Expression system for standard: E Coli, Immunogen sequence: H20-V223) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SAP amount of sample captured in plate.

NCBI and Uniprot Product Information

• NCBI GI #: 4502133
• NCBI GeneID: 325
• NCBI Accession #: NP_001630.1
• NCBI GenBank Nucleotide #: NM_001639.3
• UniProt Accession #: P02743
• Molecular Weight: 25,387 Da
• NCBI Official Full Name: serum amyloid P-component
• NCBI Official Synonym Full Names: amyloid P component, serum
• NCBI Official Symbol: APCS
• NCBI Official Synonym Symbols: SAP; PTX2; HEL-S-92n
• NCBI Protein Information: serum amyloid P-component
• UniProt Protein Name: Serum amyloid P-component
• UniProt Gene Name: APCS
• UniProt Synonym Gene Names: PTX2; SAP

NCBI Description

The protein encoded by this gene is a glycoprotein, belonging to the pentraxin family of proteins, which has a characteristic pentameric organization. These family members have considerable sequence homology which is thought to be the result of gene duplication. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo. [provided by RefSeq, Sep 2008]

Uniprot Description

APCS: Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin. SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits. Belongs to the pentaxin family.

Protein type: Chaperone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q23.2

Cellular Component: extracellular matrix; extracellular region; extracellular space; nucleus

Molecular Function: calcium ion binding; complement component C1q binding; unfolded protein binding; virion binding

Biological Process: acute-phase response; cellular protein metabolic process; chaperone-mediated protein complex assembly; innate immune response; negative regulation of acute inflammatory response; negative regulation of monocyte differentiation; negative regulation of viral reproduction; negative regulation of virion penetration into host cell; protein folding