Western Blot (WB)
(Anti-NSFL1C rabbit polyclonal antibody at 1:500 dilution Lane A: 293T Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: H1299 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:41 kDa)
Rabbit anti-Human NSFL1C Polyclonal Antibody | anti-NSFL1C antibody
Anti-NSFL1C Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
NSFL1C; Polyclonal Antibody; Anti-NSFL1C Antibody; Rabbit Polyclonal; NSFL1C Antibody; Rabbit PAb; Antigen Affinity Purified; NSFL1 (p97) cofactor (p47); Anti-dJ776F14.1 Antibody; Anti-P47 Antibody; Anti-UBX1 Antibody; Anti-UBXD10 Antibody; Anti-UBXN2C Antibody; anti-NSFL1C antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human NSFL1C
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-NSFL1C antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
ICC/IF: 1:100-1:500
IP: 1-5uL/mg of lysate
ICC/IF: 1:100-1:500
IP: 1-5uL/mg of lysate
Immunogen
E Coli-derived Human NSFL1C fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human NSFL1C fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-NSFL1C rabbit polyclonal antibody at 1:500 dilution Lane A: 293T Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: H1299 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:41 kDa)
Western Blot (WB)
(Anti-NSFL1C rabbit polyclonal antibody at 1:500 dilution Lane A: 293T Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: H1299 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:41 kDa)
Immunofluorescence (IF)
(Immunofluorescence staining of NSFL1C in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NSFL1C polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus / Cytoplasm and Cell membrane.)
Immunofluorescence (IF)
(Immunofluorescence staining of NSFL1C in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NSFL1C polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus / Cytoplasm and Cell membrane.)
Immunoprecipitation (IP)
(NSFL1C was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate 4 uL anti-NSFL1C rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NSFL1C rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 41 kDa Observed band size :46 kDa)
Immunoprecipitation (IP)
(NSFL1C was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate 4 uL anti-NSFL1C rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NSFL1C rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 41 kDa Observed band size :46 kDa)
Related Product Information for anti-NSFL1C antibody
NSFL1C (NSFL1 Cofactor) is a Protein Coding gene. The NSFL1C gene, located on 20p13, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, and rice. N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. The related pathways of NSFL1C are Protein processing in the endoplasmic reticulum.
References
Zhang X, et al. (2015) Altered cofactor regulation with disease-associated p97/vcp mutations. Proc Natl Acad Sci U S A 112 (14): E1705-1714.Park ES, et al. (2017) The opposite role of two uba-ubx containing proteins, p47 and saks1 in the degradation of a single erad substrate, alpha-tcr. Mol Cell Biochem 425 (1-2): 37-45.Kondo H, et al. (1997) P47 is a cofactor for p97-mediated membrane fusion. Nature 388 (6637): 75-78.
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Product Notes
The NSFL1C (Catalog #AAA8107993) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NSFL1C Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NSFL1C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 ICC/IF: 1:100-1:500 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the NSFL1C for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NSFL1C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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