Testing Data
Tryptophan Hydroxylase Polyclonal Antibody | anti-TPH1 antibody
Anti-Tryptophan Hydroxylase
Applications
Dot Blot, Immunohistochemistry, Western Blot
Purity
Affinity Purified Sheep polyclonal antibody.
Synonyms
Tryptophan Hydroxylase; Polyclonal Antibody; Anti-Tryptophan Hydroxylase; anti-TPH1 antibody
Clonality
Polyclonal
Specificity
Specific for the ~55k TPH protein in Western blots of human dorsal Raphe nucleus. Immunolabeling has been demonstrated in rat brain tissues. Based upon the relatively high degree of homology of tryptophan hydroxylase, the antibodies should cross-react with other mammalian species.
Purity/Purification
Affinity Purified Sheep polyclonal antibody.
Form/Format
100 ul in 150 mM NaCl, 10 mM HEPES (pH 7.5), 100 ug per ml BSA and 5O% glycerol. Adequate amount of material to conduct 1O-mini Western blots.
Sequence Length
444
Applicable Applications for anti-TPH1 antibody
Dot blots (DB) Immunohistochemistry (IHC) and Western blots (WB).
Application Notes
DB: 1:1000
IHC: 1:1000
WB: 1:1000
Quality Control Tests: Western blots performed on each lot.
IHC: 1:1000
WB: 1:1000
Quality Control Tests: Western blots performed on each lot.
Preparation and Storage
Ships on blue ice. For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.
Testing Data
Testing Data
Related Product Information for anti-TPH1 antibody
Affinity purified sheep polyclonal antibody
References
• Haycock JW, Kumer SC, Lewis DA, Vrana KE, Stockmeier CA (2002) A monoclonal antibody to tryptophan hydroxylase: applications and identification of the epitope. J Neurosci Methods 114:205-212.
• Martinez A, Knappskog PM, Haavik J (2001) A structural approach into human tryptophan hydroxylase and its implications for the regulation of serotonin biosynthesis. Curr Med Chem 8:1077-1091.
• Martinez A, Knappskog PM, Haavik J (2001) A structural approach into human tryptophan hydroxylase and its implications for the regulation of serotonin biosynthesis. Curr Med Chem 8:1077-1091.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
tryptophan 5-hydroxylase 2
NCBI Official Symbol
TPH2
NCBI Protein Information
tryptophan 5-hydroxylase 2
UniProt Protein Name
Tryptophan 5-hydroxylase 1
Protein Family
UniProt Gene Name
TPH1
UniProt Synonym Gene Names
TPH
UniProt Entry Name
TPH1_RABIT
Uniprot Description
Catalytic activity: L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.
Cofactor: Fe2+ ion.
Pathway: Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
Subunit structure: Homotetramer. Ref.3
Sequence similarities: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.Contains 1 ACT domain.
Similar Products
Product Notes
The TPH1 tph1 (Catalog #AAA502127) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Tryptophan Hydroxylase can be used in a range of immunoassay formats including, but not limited to, Dot blots (DB) Immunohistochemistry (IHC) and Western blots (WB). DB: 1:1000 IHC: 1:1000 WB: 1:1000 Quality Control Tests: Western blots performed on each lot. Researchers should empirically determine the suitability of the TPH1 tph1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tryptophan Hydroxylase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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