Sheep anti-Rat Dynamin (Ser778) Polyclonal Antibody | anti-DNM1 antibody

Anti-Phospho-Ser778 Dynamin

Cat. Num. AAA502029

• Gene Names: Dnm1; Dnm
• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from sheep serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: Dynamin (Ser778); Polyclonal Antibody; Anti-Phospho-Ser778 Dynamin; anti-DNM1 antibody

• Host: Sheep
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~95k dynamin protein phosphorylated at Ser778. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by ?-phosphatase treatment.
• Purity/Purification: Affinity Purified (Prepared from sheep serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 851

• Applicable Applications for anti-DNM1 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser778 of rat dynamin.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser778 conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, chicken, human and mouse
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-DNM1 antibody

Affinity purified sheep polyclonal antibody

References

• Cousin MA, Tan TC, Robinson PJ (2001) Protein phosphorylation is required for endocytosis in nerve terminals: potential role for the dephosphins dynamin I and synaptojanin, but not AP180 or amphiphysin. J Neurochem 76:105-116.
• Graham ME, O'Callaghan DW, McMahon HT, Burgoyne RD (2002) Dynamin-dependent and dynamin-independent processes contribute to the regulation of single vesicle release kinetics and quantal size. Proc Natl Acad Sci USA 99:7124-7129.
• Tan TC, Valova VA, Malladi CS, Graham ME, Berven LA, Jupp OJ, Hansra G, McClure SJ, Sarcevic B, Boadle RA, Larsen MR, Cousin MA, Robinson PJ (2003) cdk5 is essential for synaptic vesicle endocytosis. Nat Cell Biol 5:701-710.
• Tsuboi T, Terakawa S, Scalettar BA, Fantus C, Roder J, Jeromin A (2002) Sweeping model of dynamin activity - Visualization of coupling between exocytosis and endocytosis under an evanescent wave microscope with green fluorescent proteins. J Biol Chem 277:15957-15961.
• Clayton EL, Sue N, Smillie KJ, O'Leary T, Bache N, Cheung G, Cole AR, Wyllie DJ, Sutherland C, Robinson PJ, Cousin MA. (2010) Dynamin I phosphorylation by GSK3 controls activity-dependent bulk endocytosis of synaptic vesicles. Nat Neurosci. 13(7):845-51.

NCBI and Uniprot Product Information

• NCBI GI #: 18093102
• NCBI GeneID: 140694
• NCBI Accession #: NP_542420.1
• NCBI GenBank Nucleotide #: NM_080689.4
• UniProt Accession #: P21575
• Molecular Weight: 95
• NCBI Official Full Name: dynamin-1
• NCBI Official Synonym Full Names: dynamin 1
• NCBI Official Symbol: Dnm1
• NCBI Official Synonym Symbols: Dnm
• NCBI Protein Information: dynamin-1; D100; B-dynamin; dynamin, brain
• UniProt Protein Name: Dynamin-1
• Protein Family: Dynamin
• UniProt Gene Name: Dnm1
• UniProt Synonym Gene Names: Dnm
• UniProt Entry Name: DYN1_RAT

NCBI Description

mediates GTP hydrolysis; plays a role in receptor mediated endocytosis; required for agonist-induced internalization of the epidermal growth factor receptor [RGD, Feb 2006]

Uniprot Description

Function: Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis

By similarity.

Catalytic activity: GTP + H2O = GDP + phosphate.

Subunit structure: Interacts with CAV1 and SH3GLB1. Interacts with SNX9. Interacts with SNX33 (via SH3 domain)

By similarity. Interacts with PHOCN. Interacts with PACSIN1, PACSIN2 and PACSIN3

By similarity. Binds SH3GL1, SH3GL2 and SH3GL3. Interacts with MYO1E (via SH3 domain). Ref.6 Ref.7 Ref.10

Subcellular location: Cytoplasm Ref.8. Cytoplasm › cytoskeleton. Note: Microtubule-associated. Isoform-specific localization. Ref.8Isoform 2: Cytoplasm Ref.8. Isoform 6: Cytoplasm Ref.8. Golgi apparatus Ref.8.

Tissue specificity: Brain-specific (peripheral sensory neurons). Ref.8

Developmental stage: Expressed in neurons after maturation.

Post-translational modification: Constitutively phosphorylated by CDK5 in nerve terminals and dephosphorylated by calcineurin when synaptic vesicle endocytosis (SVE) is stimulated by depolarization-dependent calcium influx. Ref.3 Ref.9 Ref.11

Sequence similarities: Belongs to the dynamin family.Contains 1 GED domain.Contains 1 PH domain.

Caution: Absence of tyrosine and threonine phosphorylation is demonstrated in Ref.3. However, tyrosine and threonine phosphorylation may still occur in different experimental conditions.