Rabbit Potassium Chloride Cotransporter (KCC2) (Ser940) Polyclonal Antibody | anti-KCC2 antibody

Anti-Phospho-Ser940 Potassium Chloride Cotransporter (KCC2)

Cat. Num. AAA502206

• Gene Names: Slc12a5; Kcc2
• Reactivity: Bovine, Canine, Human, Mouse and Non-Human Primate
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and non-phosphopeptide affinity columns.)
• Synonyms: Potassium Chloride Cotransporter (KCC2) (Ser940); Polyclonal Antibody; Anti-Phospho-Ser940 Potassium Chloride Cotransporter (KCC2); anti-KCC2 antibody

• Host: Rabbit
• Reactivity: Bovine, Canine, Human, Mouse and Non-Human Primate
• Clonality: Polyclonal
• Specificity: Specific for the ~135k KCC2 protein phosphorylated at Ser940. Immunolabeling of the KCC2 protein band is blocked by the phospho-peptide used as antigen but not by the corresponding dephosphopeptide.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and non-phosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 1116

• Applicable Applications for anti-KCC2 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Species Tested: Mouse, Rat
• Biological Significance: KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser940 increased the cell surface stability of KCC2 in this system by decreasing it's rate of internalization from the plasma membrane (Lee et al., 2007).
• Preparation and Storage: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without without freeze/thawing due to the presence of 50% glycerol. Stock solutions for a minimum of 1 year at -20°C.

Testing Data

Related Product Information for anti-KCC2 antibody

Affinity purified rabbit polyclonal antibody

References

• Lee HH, Walker JA, Williams JR, Goodier RJ, Payne JA, Moss SJ (2007) Direct protein kinase C-dependent phosphorylation regulates the cell surface stability and activity of the potassium chloride cotransporter KCC2. J Biol Chem. 2007 Oct 12;282(41):29777-84
• Lee HH, Deeb TZ, Walker JA, Davies PA, Moss SJ (2011) NMDA receptor activity downregulates KCC2 resulting in depolarizing GABAA receptor-mediated currents. Nat Neurosci. Jun;14(6):736-43

NCBI and Uniprot Product Information

• NCBI GI #: 19705463
• NCBI GeneID: 171373
• NCBI Accession #: NP_599190.1
• NCBI GenBank Nucleotide #: NM_134363.1
• UniProt Accession #: Q63633; A7Y821
• Molecular Weight: 135kDa
• NCBI Official Full Name: solute carrier family 12 member 5
• NCBI Official Synonym Full Names: solute carrier family 12 (potassium-chloride transporter), member 5
• NCBI Official Symbol: Slc12a5
• NCBI Official Synonym Symbols: Kcc2
• NCBI Protein Information: solute carrier family 12 member 5; rKCC2; K-Cl cotransporter 2; neuronal K-Cl cotransporter; solute carrier family 12, member 5; neuronal-specific K-Cl cotransporter; furosemide-sensitive K-Cl cotransporter; electroneutral potassium-chloride cotransporter 2
• UniProt Protein Name: Solute carrier family 12 member 5
• UniProt Gene Name: Slc12a5
• UniProt Synonym Gene Names: Kcc2; rKCC2
• UniProt Entry Name: S12A5_RAT

NCBI Description

neuronal specific potassium-chloride cotransporter isoform; may play a role in response to neuronal injury [RGD, Feb 2006]

Uniprot Description

Function: Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl- homeostasis in neurons. Necessary for the ontogenic change in response to gamma-aminobutyric acid (GABA) from depolarization to hyperpolarization during neuronal development. Ref.3

Enzyme regulation: Inhibited by WNK3

By similarity.

Subunit structure: Homomultimer and heteromultimer with other K-Cl cotransporters. Interacts with AP2A1

By similarity. Ref.4

Subcellular location: Membrane; Multi-pass membrane protein.

Tissue specificity: Highly expressed in brain. Not detected in other tissues. Highly expressed in pyramidal neurons and in neurons throughout the cortex, hippocampus, the granular layer of the cerebellum and in groups of neurons throughout the brainstem. Barely detectable in dorsal-root ganglions.

Developmental stage: Detected in thalamus, but not in hippocampus and neocortex at E20. At birth barely detectable in hippocampus. Expression increases steeply from day 5 to day 9 and then stabilizes at adult levels.

Sequence similarities: Belongs to the SLC12A transporter family.