Rabbit anti-Rat Gap-43 (Ser41) Polyclonal Antibody | anti-GAP43 antibody

Anti-Phospho-Ser41 Gap-43

Cat. Num. AAA502060

• Gene Names: Gap43; Basp2
• Reactivity: Rat
• Applications: Dot Blot, Immunohistochemistry, Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: Gap-43 (Ser41); Polyclonal Antibody; Anti-Phospho-Ser41 Gap-43; anti-GAP43 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~50k Gap-43 phosphorylated at Ser⁴¹ in Western blots of rat brain lysate. In some tissues the antibody also recognized a higher molecular weigth band that is also recognized by the pan Gap-43 antibody and this band may be a Gap-43 aggregate or oligomer. Immunolabeling of the Gap-43 protein is blocked by the Ser ⁴¹ phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. Human, mouse and rat have 100% amino acid sequence identity with for the antigen used to raise the antibody.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 226

• Applicable Applications for anti-GAP43 antibody: Dot blots (DB) and Western blots (WB). Immunohistochemistry (IHC) application has not yet been determined.
• Application Notes: DB: 1:1000; WB: 1:1000
• Quality Control Tests: Western blots performed on each lot.
• Preparation and Storage: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.

Testing Data

Related Product Information for anti-GAP43 antibody

Affinity purified rabbit polyclonal antibody

References

• Benowitz LI, Routtenberg A (1997) Gap-43: An intrinsic determinant of neuronal development and plasticit. Trends Neurosci 20:84-91.
• He, Q, Dent, EW, Meiri, KF (1997) Modulation of actin filament behavior by Gap-43 (neuromodulin) is dependent on the phosphorylation status of serine 41, the protein kinase C site. J Neurosci 17:3515-3524.
• Hulo S, Alberi, S, Laux T, Muller D, Caroni P (2002) A point mutant of Gap-43 induces enhanced short-term and long-term hippocampal plasticity. Eur J Neurosci 15:1976-1982.
• Strittmatter SM, Fankhauser C, Huang PL, Mashimo H, Fishman MC (1995) Neuronal path finding is abnormal in mice lacking the neuronal growth cone protein Gap-43," Cell 80:445-452.
• Rayudu Gopalakrishna, Usha Gundimeda, Jason Eric Schiffman, and Thomas H. McNeill (2008) A Direct Redox Regulation of Protein Kinase C Isoenzymes Mediates Oxidant-induced Neuritogenesis in PC12 Cells J. Biol. Chem., May 2008; 283: 14430 - 14444.

NCBI and Uniprot Product Information

• NCBI GI #: 8393415
• NCBI GeneID: 29423
• NCBI Accession #: NP_058891.1
• NCBI GenBank Nucleotide #: NM_017195.3
• UniProt Accession #: P07936; A0JPM4
• NCBI Official Full Name: neuromodulin
• NCBI Official Synonym Full Names: growth associated protein 43
• NCBI Official Symbol: Gap43
• NCBI Official Synonym Symbols: Basp2
• NCBI Protein Information: neuromodulin; protein F1; growth-associated protein 43; axonal membrane protein GAP-43; growth accentuating protein 43; brain abundant, membrane attached signal protein 2
• UniProt Protein Name: Neuromodulin
• Protein Family: Neuromodulin
• UniProt Gene Name: Gap43
• UniProt Entry Name: NEUM_RAT

NCBI Description

may play a role in neurite outgrowth and nerve regeneration [RGD, Feb 2006]

Uniprot Description

Function: This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction

By similarity.

Subunit structure: Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN

By similarity. Binds calmodulin with a greater affinity in the absence of Ca2+ than in its presence

By similarity.

Subcellular location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection › growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction › synapse. Cell projection › filopodium membrane; Peripheral membrane protein

By similarity. Note: Cytoplasmic surface of growth cone and synaptic plasma membranes.

Post-translational modification: Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2

By similarity.Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Ref.7 Ref.8 Ref.9

Sequence similarities: Belongs to the neuromodulin family.Contains 1 IQ domain.