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Immunohistochemistry (IHC) (Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

Rabbit anti-Human HSPA9 Polyclonal Antibody | anti-HSPA9 antibody

HSPA9, CT (GRP75, Stress-70 Protein, Mitochondrial, 75kD Glucose-regulated Protein, GRP-75, Heat Shock 70kD Protein 9, Mortalin, MOT, Peptide-binding Protein 74, PBP74, HSPA9B)

Gene Names
HSPA9; CSA; MOT; MOT2; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Synonyms
HSPA9; Polyclonal Antibody; CT (GRP75; Stress-70 Protein; Mitochondrial; 75kD Glucose-regulated Protein; GRP-75; Heat Shock 70kD Protein 9; Mortalin; MOT; Peptide-binding Protein 74; PBP74; HSPA9B); Anti -HSPA9; anti-HSPA9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GRP75. Species sequence homology: mouse and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-HSPA9 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Dilution: ELISA: 1:5000
Western Blot: 1:500-1:1000
Immunofluorescence: 1:100-1:500
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Immunogen
Synthetic peptide corresponding to human GRP75.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC) (Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of extracts from COS7 cells, using GRP75 Antibody. The lane on the right is treated with the synthesized peptide.)

Western Blot (WB) (Western blot analysis of extracts from COS7 cells, using GRP75 Antibody. The lane on the right is treated with the synthesized peptide.)

Immunofluorescence (IF)

(Immunofluorescence analysis of COS7 cells, using GRP75 Antibody. The picture on the right is treated with the synthesized peptide.)

Immunofluorescence (IF) (Immunofluorescence analysis of COS7 cells, using GRP75 Antibody. The picture on the right is treated with the synthesized peptide.)
Related Product Information for anti-HSPA9 antibody
Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
Product Categories/Family for anti-HSPA9 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
73,680 Da
NCBI Official Full Name
HSPA9 protein
NCBI Official Synonym Full Names
heat shock 70kDa protein 9 (mortalin)
NCBI Official Symbol
HSPA9
NCBI Official Synonym Symbols
CSA; MOT; MOT2; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75
NCBI Protein Information
stress-70 protein, mitochondrial; mortalin-2; p66-mortalin; mortalin, perinuclear; heat shock 70kD protein 9B; peptide-binding protein 74; 75 kDa glucose-regulated protein
UniProt Protein Name
Stress-70 protein, mitochondrial
Protein Family
UniProt Gene Name
HSPA9
UniProt Synonym Gene Names
GRP75; HSPA9B; mt-HSP70; GRP-75; MOT; PBP74
UniProt Entry Name
GRP75_HUMAN

NCBI Description

This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.[provided by RefSeq, May 2010]

Uniprot Description

Function: Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.

Subunit structure: Interacts with FXN. Interacts with HSCB. Component of the MINOS/MitOS complex, that includes IMMT, HSPA9 and CHCHD3 and associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2. Interacts with DNLZ, the interaction is required to prevent self-aggregation. Ref.13 Ref.15 Ref.18 Ref.20

Subcellular location: Mitochondrion. Nucleus › nucleolus Ref.19.

Sequence similarities: Belongs to the heat shock protein 70 family.

Research Articles on HSPA9

Similar Products

Product Notes

The HSPA9 hspa9 (Catalog #AAA646132) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSPA9, CT (GRP75, Stress-70 Protein, Mitochondrial, 75kD Glucose-regulated Protein, GRP-75, Heat Shock 70kD Protein 9, Mortalin, MOT, Peptide-binding Protein 74, PBP74, HSPA9B) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSPA9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Dilution: ELISA: 1:5000 Western Blot: 1:500-1:1000 Immunofluorescence: 1:100-1:500 Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml. Researchers should empirically determine the suitability of the HSPA9 hspa9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPA9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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