Rabbit anti-Human MAD2L1 / MAD2 Polyclonal Antibody | anti-MAD2L1 antibody

Rabbit Polyclonal to Human MAD2L1 / MAD2

Cat. Num. AAA240551

• Gene Names: MAD2L1; MAD2; HSMAD2
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: MAD2L1 / MAD2; Polyclonal Antibody; Rabbit Polyclonal to Human MAD2L1 / MAD2; Anti-MAD2L1 / MAD2 Antibody (aa3-13) IHC-plus; MAD2L1; HSMAD2; MAD2; MAD2-like protein 1; Human MAD2L1; anti-MAD2L1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Amino acid residues 3-13 of Human MAD2L1 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 205

• Applicable Applications for anti-MAD2L1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:2000 - 1:10000), IF, IHC-P (2.5 ug/ml), WB (1:500 - 1:2000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human MAD2L1.
• Immunogen Type: Synthetic peptide
• Immunogen: MAD2L1 / MAD2 antibody was raised against synthetic peptide from human MAD2L1.
• Antigen Modification: aa3-13
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MAD2L1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-MAD2L1 Antibody - Western Blot. Western blot of Affinity Purified anti-MAD2L1 antibody shows detection of a predominant band at ~24 kD corresponding to MAD2L1 (arrowhead) present in Jurkat (lane 1) and HeLa (lane 2) whole cell lysates using the 800 nm channel (green). The identity of the higher molecular weight bands is unknown, although they may represent complexes of MAD2L1 with related binding proteins. Specific band reactivity is blocked when the antibody is pre-incubated with immunizing peptide (lanes 4 and 5 respectively) which completely blocks antibody staining. ~35 ug of lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1200. Incubation was 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MXHu (for 45 min at room temperature. Molecular weight markers were used for size comparison using the 700 nm channel (lane 3). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)

NCBI and Uniprot Product Information

• NCBI GI #: 4505067
• NCBI GeneID: 4085
• NCBI Accession #: NP_002349.1
• NCBI GenBank Nucleotide #: NM_002358.3
• UniProt Accession #: Q13257; Q53F56; Q548X9; Q6IRW7; Q8IZX3
• Molecular Weight: 10,335 Da
• NCBI Official Full Name: mitotic spindle assembly checkpoint protein MAD2A
• NCBI Official Synonym Full Names: MAD2 mitotic arrest deficient-like 1 (yeast)
• NCBI Official Symbol: MAD2L1
• NCBI Official Synonym Symbols: MAD2; HSMAD2
• NCBI Protein Information: mitotic spindle assembly checkpoint protein MAD2A; MAD2-like protein 1; mitotic arrest deficient 2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
• UniProt Protein Name: Mitotic spindle assembly checkpoint protein MAD2A
• Protein Family: Mitotic spindle assembly checkpoint protein
• UniProt Gene Name: MAD2L1
• UniProt Synonym Gene Names: MAD2; HsMAD2; MAD2-like protein 1
• UniProt Entry Name: MD2L1_HUMAN

NCBI Description

MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008]

Uniprot Description

MAD2L1: required for the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. Inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. Interacts with Cdc20, MAD2L1BP and with ADAM17/TACE

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: kinetochore; spindle pole; perinuclear region of cytoplasm; nuclear pore; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; protein homodimerization activity

Biological Process: negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic sister chromatid segregation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; negative regulation of mitotic cell cycle; mitotic cell cycle; negative regulation of protein catabolic process; negative regulation of apoptosis