Rabbit anti-Human PRL3 Polyclonal Antibody | anti-PRL3 antibody

PRL3, CT (PTP4A3, PRL-3, PRL-R, Protein Tyrosine Phosphatase Type IVA, Member 3, Protein-tyrosine Phosphatase 4a3, Protein-tyrosine Phosphatase Of Regenerating Liver 3) (AP)

Cat. Num. AAA6495851

• Gene Names: PTP4A3; PRL3; PRL-3; PRL-R
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PRL3; Polyclonal Antibody; CT (PTP4A3; PRL-3; PRL-R; Protein Tyrosine Phosphatase Type IVA; Member 3; Protein-tyrosine Phosphatase 4a3; Protein-tyrosine Phosphatase Of Regenerating Liver 3) (AP); anti-PRL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRL3.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PRL3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PRL3 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PTP4A3 C-term in MCF-7 and 293 cell line lysates (35ug/lane). PTP4A3 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-PRL3 antibody

Antibodies; Enzymes; Phosphatase

References

1. Wu, X., et al., Am. J. Pathol. 164(6):2039-2054 (2004). 2. Kozlov, G., et al., J. Biol. Chem. 279(12):11882-11889 (2004). 3. Bardelli, A., et al., Clin. Cancer Res. 9(15):5607-5615 (2003). 4. Matter, W.F., et al., Biochem. Biophys. Res. Commun. 283(5):1061-1068 (2001). 5. Saha, S., et al., Science 294(5545):1343-1346 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 14589856
• NCBI GeneID: 11156
• NCBI Accession #: NP_116000
• NCBI GenBank Nucleotide #: NM_032611.2
• UniProt Accession #: O75365; Q8IVN5; Q99849; Q9BTW5
• Molecular Weight: 21.6 kDa (193aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: protein tyrosine phosphatase type IVA 3 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase type IVA, member 3
• NCBI Official Symbol: PTP4A3
• NCBI Official Synonym Symbols: PRL3; PRL-3; PRL-R
• NCBI Protein Information: protein tyrosine phosphatase type IVA 3
• UniProt Protein Name: Protein tyrosine phosphatase type IVA 3
• UniProt Gene Name: PTP4A3
• UniProt Synonym Gene Names: PRL3; PRL-3
• UniProt Entry Name: TP4A3_HUMAN

NCBI Description

This gene encodes a member of the protein-tyrosine phosphatase family. Protein tyrosine phosphatases are cell signaling molecules that play regulatory roles in a variety of cellular processes. Studies of this class of protein tyrosine phosphatase in mice demonstrates that they are prenylated in vivo, suggesting their association with cell plasma membrane. The encoded protein may enhance cell proliferation, and overexpression of this gene has been implicated in tumor metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

PTP4A3: Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis. May be involved in the progression of cardiac hypertrophy by inhibiting intracellular calcium mobilization in response to angiotensin II. Belongs to the protein-tyrosine phosphatase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: early endosome; plasma membrane

Molecular Function: prenylated protein tyrosine phosphatase activity

Biological Process: endothelial cell migration