Western Blot (WB)
(Western Blot analysis of extracts from C2C12 cells using MBS621192.)
Rabbit anti-Human, Mouse Insulin Regulated Aminopeptidase Polyclonal Antibody | anti-LNPEP antibody
Insulin Regulated Aminopeptidase (IRAP, Placental Leucine Aminopeptidase, PLAP, Leucyl Cystinyl Aminopeptidase, LNPEP, Oxytocinase, Otase, Vesicle Protein of 165kD, vp165)
Gene Names
LNPEP; IRAP
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Insulin Regulated Aminopeptidase; Polyclonal Antibody; Insulin Regulated Aminopeptidase (IRAP; Placental Leucine Aminopeptidase; PLAP; Leucyl Cystinyl Aminopeptidase; LNPEP; Oxytocinase; Otase; Vesicle Protein of 165kD; vp165); Anti -Insulin Regulated Aminopeptidase (IRAP; anti-LNPEP antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Recognizes Human IRAP. Species crossreactivity
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied in 10 mM sodium HEPES (pH 7.5), 150mM NaCl, 100ug/ml BSA and 50% glycerol.
Applicable Applications for anti-LNPEP antibody
Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes
Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence (IF-IC).
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:50.
Immunofluorescence (IF-IC): 1:50
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:50.
Immunofluorescence (IF-IC): 1:50
Immunogen
Synthetic peptide corresponding to the sequence of human IRAP protein (KLH-coupled).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot analysis of extracts from C2C12 cells using MBS621192.)
Western Blot (WB)
(Western Blot analysis of extracts from C2C12 cells using MBS621192.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of differentiated 3T3-L1 cells using MBS621192(red). Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of differentiated 3T3-L1 cells using MBS621192(red). Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).)
Related Product Information for anti-LNPEP antibody
IRAP (also known as LNPEP) was originally described as an insulin-responsive aminopeptidase found in GLUT4-containing vesicles (1), but later found to be a critical enzyme that regulates the expression and activity of several essential hormones and regulatory proteins, including the GLUT4 transporter (2,3). This membrane associated, zinc-dependent cystinyl aminopeptidase acts as both a receptor for angiotensin IV as well as the enzyme that catalyzes the synthesis of this essential hormone from its angiotensinogen precursor (4). IRAP catalyzes the hydrolysis of several peptide hormones, including oxytocin and vasopressin (3). Abnormal IRAP expression or activity is associated with several forms of cancer in humans, including renal and endometrial cancer (5,6).
Product Categories/Family for anti-LNPEP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
117,368 Da
NCBI Official Full Name
insulin-regulated aminopeptidase
NCBI Official Symbol
LNPEP
NCBI Official Synonym Symbols
IRAP
NCBI Protein Information
leucyl-cystinyl aminopeptidase; insulin-regulated aminopeptidase
UniProt Protein Name
Insulin-regulated aminopeptidase
Protein Family
UniProt Gene Name
IRAP
UniProt Entry Name
A7WK50_PIG
Similar Products
Product Notes
The LNPEP irap (Catalog #AAA621192) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Insulin Regulated Aminopeptidase (IRAP, Placental Leucine Aminopeptidase, PLAP, Leucyl Cystinyl Aminopeptidase, LNPEP, Oxytocinase, Otase, Vesicle Protein of 165kD, vp165) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Insulin Regulated Aminopeptidase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF). Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence (IF-IC). Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Immunoprecipitation: 1:50. Immunofluorescence (IF-IC): 1:50. Researchers should empirically determine the suitability of the LNPEP irap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Insulin Regulated Aminopeptidase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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