Rabbit anti-Human Parvin, alpha Polyclonal Antibody | anti-PARVA antibody

Parvin, alpha

Cat. Num. AAA613611

• Gene Names: PARVA; MXRA2; CH-ILKBP; FLJ10793; FLJ12254; FLJ25106; FLJ46049
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by peptide affinity chromatography.
• Synonyms: Parvin; alpha; Polyclonal Antibody; Anti -Parvin; anti-PARVA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total -parvin protein.
• Purity/Purification: Purified by peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 g/ml BSA and 50% glycerol.

• Applicable Applications for anti-PARVA antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic peptide (KLH-coupled) corresponding to residues near the amino terminus of human alpha-parvin.
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from various cell types using MBS613611. In lane 2, HeLa cells were isolated in mitosis by mitotic shake-off following nocodozole treatment (100 ng/mL for 4 hours).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HeLa cells using MBS613611 (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)

Related Product Information for anti-PARVA antibody

The extracellular matrix (ECM) is a complex structure of secreted macromolecules surrounding mammalian organs and tissues. Controlled interactions between cells and the ECM are important in proliferation, migration, survival, polarity and differentiation. Cells contact the ECM primarily through heterodimeric integral membrane proteins called integrins. Integrins connect the ECM to the cytoskeleton, and therefore the cell signaling machinery, through protein complexes called focal adhesions (1). The ILK/PINCH/Parvin (IPP) complex is composed of three highly conserved proteins recruited to sites of ECM contact as pre-assembled structures. The IPP acts at the interface of the integrin/actin connection to regulate formation of focal adhesions and integrin signaling. All three proteins contain multiple protein binding domains allowing them to function as adaptor proteins in the formation of focal adhesions. ILK (integrin-linked kinase) also has a catalytic (protein Ser/Thr kinase) domain, but may or may not function as a kinase in vivo. Roles for IPP proteins outside of the IPP complex have been proposed, including regulation of gene expression (2,3). The parvin family consists of 3 members, -parvin/actopaxin, -parvin/affixin and -parvin. -parvin and -parvin are expressed ubiquitously, while expression of -parvin is restricted to haematopoietic cells (4). -parvin binds to F-actin both directly and via interaction with the focal adhesion protein paxillin (5). -parvin regulates cell spreading and motility through interactions with the cofilin kinase TESK1 (6), and with the GTPase activating protein CdGAP (7). Phosphorylation of -parvin during mitosis may have a role in the regulation of actin dynamics during the cell cycle (8).

Product Categories/Family for anti-PARVA antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 16876864
• NCBI GeneID: 55742
• UniProt Accession #: Q9NVD7; Q96C85; Q9HA48
• Molecular Weight: 42,244 Da
• NCBI Official Full Name: Parvin, alpha
• NCBI Official Synonym Full Names: parvin, alpha
• NCBI Official Symbol: PARVA
• NCBI Official Synonym Symbols: MXRA2; CH-ILKBP; FLJ10793; FLJ12254; FLJ25106; FLJ46049
• NCBI Protein Information: alpha-parvin; actopaxin; OTTHUMP00000230952; matrix-remodeling-associated protein 2; calponin-like integrin-linked kinase-binding protein
• UniProt Protein Name: Alpha-parvin
• Protein Family: Alpha-parvin
• UniProt Gene Name: PARVA
• UniProt Synonym Gene Names: MXRA2
• UniProt Entry Name: PARVA_HUMAN

NCBI Description

This gene encodes a member of the parvin family of actin-binding proteins. Parvins are associated with focal contacts and contain calponin homology domains that bind to actin filaments. The encoded protein is part of the integrin-linked kinase signaling complex and plays a role in cell adhesion, motility and survival. [provided by RefSeq]

Uniprot Description

PARVA: Probably plays a role in the regulation of cell adhesion and cytoskeleton organization. Plays a role in ciliogenesis. Interacts with TGFB1I1. Interacts with integrin-linked protein kinase and probably with actin and the LD1 and LD4 motifs of PXN. Interacts with ARHGAP31. Widely expressed, with highest levels in heart, skeletal muscle, kidney and liver. Belongs to the parvin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p15.3

Cellular Component: focal adhesion; lamellipodium; cytoplasm; plasma membrane; Z disc; nucleus; cytosol; actin cytoskeleton

Molecular Function: protein binding; actin binding

Biological Process: heterotypic cell-cell adhesion; regulation of cell shape; actin cytoskeleton reorganization; establishment and/or maintenance of cell polarity; sprouting angiogenesis