Testing Data
PI4KA recombinant protein
PI4KA, Active
Gene Names
PI4KA; PIK4CA; PMGYCHA; pi4K230; PI4K-ALPHA
Applications
Kinase Assay
Synonyms
PI4KA; Active; PI4KA recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
Full length
Sequence Length
854
Applicable Applications for PI4KA recombinant protein
Kinase Assay
Species
Human
Tag
GST tag
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for PI4KA recombinant protein
Description: Recombinant full-length human PI4KA was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PI4KA encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. The protein encoded by PI4KA gene is a type III enzyme that is not inhibited by adenosine. PI4KA is highly expressed in placenta and brain (1). PI4KA is associated with the membranes of Golgi vesicles and vacuoles (2).
Scientific Background: PI4KA encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. The protein encoded by PI4KA gene is a type III enzyme that is not inhibited by adenosine. PI4KA is highly expressed in placenta and brain (1). PI4KA is associated with the membranes of Golgi vesicles and vacuoles (2).
Product Categories/Family for PI4KA recombinant protein
References
1. Wong, K. et.al: Cloning and characterization of a human phosphatidylinositol 4-kinase. J. Biol. Chem. 269: 28878-28884, 1994. 2. Nakagawa, T. et.al: Cloning, expression, and localization of 230-kDa phosphatidylinositol 4-kinase. J. Biol. Chem. 271: 12088-12094, 1996.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~120 kDa
NCBI Official Synonym Full Names
phosphatidylinositol 4-kinase alpha
NCBI Official Symbol
PI4KA
NCBI Official Synonym Symbols
PIK4CA; PMGYCHA; pi4K230; PI4K-ALPHA
NCBI Protein Information
phosphatidylinositol 4-kinase alpha
UniProt Protein Name
Phosphatidylinositol 4-kinase alpha
Protein Family
UniProt Gene Name
PI4KA
UniProt Synonym Gene Names
PIK4; PIK4CA; PI4-kinase alpha; PI4K-alpha; PtdIns-4-kinase alpha
NCBI Description
This gene encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. The protein encoded by this gene is a type III enzyme that is not inhibited by adenosine. [provided by RefSeq, Sep 2014]
Uniprot Description
Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate.
Research Articles on PI4KA
Similar Products
Product Notes
The PI4KA pi4ka (Catalog #AAA515594) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full length. AAA Biotech's PI4KA can be used in a range of immunoassay formats including, but not limited to, Kinase Assay. Researchers should empirically determine the suitability of the PI4KA pi4ka for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI4KA, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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