SDS-Page
(Extracts of His-tagged S139A/T421A double mutant (1) and wild-type PAK3 (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% milk- TBST buffer for one hour at RT, then incubated with MBS620041 for two hours at RT in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide)
Rabbit anti-Human PAK1,2,3 Polyclonal Antibody
PAK1,2,3, phosphorylated (Ser144/Ser141/Ser139) (p21 Activated Kinase 1,2,3)
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
PAK1; 2; 3; Polyclonal Antibody; phosphorylated (Ser144/Ser141/Ser139) (p21 Activated Kinase 1; 3); Anti -PAK1; anti-PAK1; 3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes human PAK1 (46%), PAK2, and PAK3 (46%) when phosphorylated at Ser144/Ser141/Ser139, respectively. Other species have not been tested, but are expected to react.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Applicable Applications for anti-PAK1,2,3 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000
Dilution: Western Blot: 1:1000
Immunogen
Synthetic phosphopeptide derived from a region of human PAK2 that contains serine 141. Species sequence homology: rat and rabbit.
Positive Control
His-tagged wild-type and S139A/T421A double mutant PAK3.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
SDS-Page
(Extracts of His-tagged S139A/T421A double mutant (1) and wild-type PAK3 (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% milk- TBST buffer for one hour at RT, then incubated with MBS620041 for two hours at RT in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide)
SDS-Page
(Extracts of His-tagged S139A/T421A double mutant (1) and wild-type PAK3 (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% milk- TBST buffer for one hour at RT, then incubated with MBS620041 for two hours at RT in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide)
Testing Data
(immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with Pab IgG F(ab’)2 (HRP) goat x rabbit and detected using SuperSignal ECL method. Only the phosphopeptide corresponding to MBS620041 blocks the antibody signal. Phosphorylation of the wild-type but not the mutant protein is detected by MBS620041)
Testing Data
(immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with Pab IgG F(ab’)2 (HRP) goat x rabbit and detected using SuperSignal ECL method. Only the phosphopeptide corresponding to MBS620041 blocks the antibody signal. Phosphorylation of the wild-type but not the mutant protein is detected by MBS620041)
Related Product Information for anti-PAK1,2,3 antibody
p21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are
Product Categories/Family for anti-PAK1,2,3 antibody
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Product Notes
The PAK1,2,3 (Catalog #AAA620041) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PAK1,2,3, phosphorylated (Ser144/Ser141/Ser139) (p21 Activated Kinase 1,2,3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PAK1,2,3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the PAK1,2,3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAK1,2,3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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