SDS-Page
(Peptide Competition Extracts prepared from HEK 293 cells left untreated (1) or treated with UV irradiation (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then incubated with JNK1&2 [pTpY183/185] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with: no peptide (1, 6), the phosphopeptide immunogen (2), a generic phosphothreonine-containing peptide (3), a generic phosphotyrosine-containing peptide (4), or, the non-phosphopeptide corresponding to the immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalÅe method.)
Rabbit JNK1/2 Polyclonal Antibody | anti-JKAMP antibody
JNK1/2, phosphorylated (Thr183, Tyr185) (c-Jun N-terminal Kinase 1, JNK1, JNK, c-Jun N-terminal Kinase 2, JNK2, JNK1A2, JNK21B1/2, JNK-46, JNK-55, Mitogen-activated Protein Kinase 8, MAP Kinase 8, MAPK 8, MAPK8, PRKM8, Mitogen-activated Protein Kinase 9,
Gene Names
JKAMP; C14orf100
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
JNK1/2; Polyclonal Antibody; phosphorylated (Thr183; Tyr185) (c-Jun N-terminal Kinase 1; JNK1; JNK; c-Jun N-terminal Kinase 2; JNK2; JNK1A2; JNK21B1/2; JNK-46; JNK-55; Mitogen-activated Protein Kinase 8; MAP Kinase 8; MAPK 8; MAPK8; PRKM8; Mitogen-activated Protein Kinase 9; ; Anti -JNK1/2; anti-JKAMP antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes human, mouse, and rat JNK1&2 when phosphorylated at Thr183, Tyr185. Other species of JNK1&2 have not been tested, and JNK3 (found primarily only in neuronal cell lines) has not been detected.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, 50% glycerol.
Applicable Applications for anti-JKAMP antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000
Dilution: Western Blot: 1:1000
Immunogen
Synthetic phosphopeptide corresponding to human JNK1&2 contains threonine 183 and tyrosine 185. Species sequence homology: mouse, rat, chick, nematode, and fruit fly, and in JNK3.
Positive Control
HEK 293 cells +/- UV irradiation treatment and PC12 cells +/- Sorbitol.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
SDS-Page
(Peptide Competition Extracts prepared from HEK 293 cells left untreated (1) or treated with UV irradiation (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then incubated with JNK1&2 [pTpY183/185] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with: no peptide (1, 6), the phosphopeptide immunogen (2), a generic phosphothreonine-containing peptide (3), a generic phosphotyrosine-containing peptide (4), or, the non-phosphopeptide corresponding to the immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalÅe method.)
SDS-Page
(Peptide Competition Extracts prepared from HEK 293 cells left untreated (1) or treated with UV irradiation (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then incubated with JNK1&2 [pTpY183/185] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with: no peptide (1, 6), the phosphopeptide immunogen (2), a generic phosphothreonine-containing peptide (3), a generic phosphotyrosine-containing peptide (4), or, the non-phosphopeptide corresponding to the immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalÅe method.)
Testing Data
(Peptide Competition Extracts prepared from HEK 293 cells left untreated (1) or treated with UV irradiation (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then incubated with JNK1&2 [pTpY183/185] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with: no peptide (1, 6), the phosphopeptide immunogen (2), a generic phosphothreonine-containing peptide (3), a generic phosphotyrosine-containing peptide (4), or, the non-phosphopeptide corresponding to the immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal‘ method.The data show that only the phosphopeptide corresponding to JNK1&2 [pTpY183/185] blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show JNK activation under stress.)
Testing Data
(Peptide Competition Extracts prepared from HEK 293 cells left untreated (1) or treated with UV irradiation (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then incubated with JNK1&2 [pTpY183/185] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with: no peptide (1, 6), the phosphopeptide immunogen (2), a generic phosphothreonine-containing peptide (3), a generic phosphotyrosine-containing peptide (4), or, the non-phosphopeptide corresponding to the immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal‘ method.The data show that only the phosphopeptide corresponding to JNK1&2 [pTpY183/185] blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show JNK activation under stress.)
Related Product Information for anti-JKAMP antibody
c-Jun N-terminal Kinase (JNK) is a Stress Activated Protein Kinase/Map Kinase (SAPK/MAPK) represented by three isoforms, JNK1 (~49kD), JNK2 (~55kD) and JNK3 (~54kD). JNK is dually phosphorylated and therefore fully activated by MEK4 and MEK7 on threonine 183 and tyrosine 185 within the activation loop. The JNK and p38 pathways function to modulate cellcycle, apoptotic and transcriptional responses to stress.
Product Categories/Family for anti-JKAMP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
JNK1/MAPK8-associated membrane protein
NCBI Official Synonym Full Names
JNK1/MAPK8-associated membrane protein
NCBI Official Symbol
JKAMP
NCBI Official Synonym Symbols
C14orf100
NCBI Protein Information
JNK1/MAPK8-associated membrane protein
Protein Family
Research Articles on JKAMP
Similar Products
Product Notes
The JKAMP (Catalog #AAA616079) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The JNK1/2, phosphorylated (Thr183, Tyr185) (c-Jun N-terminal Kinase 1, JNK1, JNK, c-Jun N-terminal Kinase 2, JNK2, JNK1A2, JNK21B1/2, JNK-46, JNK-55, Mitogen-activated Protein Kinase 8, MAP Kinase 8, MAPK 8, MAPK8, PRKM8, Mitogen-activated Protein Kinase 9, reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's JNK1/2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the JKAMP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JNK1/2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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