Testing Data
(Peptide Competition Extracts prepared from HEK 293 cells treated with UV irradiation were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSATBST buffer overnight at 4oC, then were incubated with p38 [pTpY180/182] antibody for two hours at RT in a 3% BSA-TBST buffer, following its prior incubation with: the peptide immunogen (1), a generic phosphothreonine-containing peptide (2), a generic phosphotyrosine-containing peptide (3), the non-phosphorylated peptide corresponding to the phosphopeptide (4), no peptide (5), the phosphopeptide derived from the corresponding region of JNK1&2 (6), and, the phosphopeptide derived from the corresponding region of ERK1&2 (7). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalÅe method.)
Rabbit anti-Human MAP Kinase p38 Polyclonal Antibody
MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 1:1000
Testing Data
(Peptide Competition Extracts prepared from HEK 293 cells treated with UV irradiation were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSATBST buffer overnight at 4oC, then were incubated with p38 [pTpY180/182] antibody for two hours at RT in a 3% BSA-TBST buffer, following its prior incubation with: the peptide immunogen (1), a generic phosphothreonine-containing peptide (2), a generic phosphotyrosine-containing peptide (3), the non-phosphorylated peptide corresponding to the phosphopeptide (4), no peptide (5), the phosphopeptide derived from the corresponding region of JNK1&2 (6), and, the phosphopeptide derived from the corresponding region of ERK1&2 (7). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalÅe method.)
Testing Data
(Peptide Competition Extracts prepared from HEK 293 cells treated with UV irradiation were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSATBST buffer overnight at 4oC, then were incubated with p38 [pTpY180/182] antibody for two hours at RT in a 3% BSA-TBST buffer, following its prior incubation with: the peptide immunogen (1), a generic phosphothreonine-containing peptide (2), a generic phosphotyrosine-containing peptide (3), the non-phosphorylated peptide corresponding to the phosphopeptide (4), no peptide (5), the phosphopeptide derived from the corresponding region of JNK1&2 (6), and, the phosphopeptide derived from the corresponding region of ERK1&2 (7). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalÅe method.)
Testing Data
(Peptide Competition Extracts prepared from HEK 293 cells treated with UV irradiation were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSATBST buffer overnight at 4oC, then were incubated with p38 [pTpY180/182] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with: the peptide immunogen (1), a generic phosphothreonine-containing peptide (2), a generic phosphotyrosine-containing peptide (3), the non-phosphorylated peptide corresponding to the phosphopeptide (4), no peptide (5), the phosphopeptide derived from the corresponding region of JNK1&2 (6), and, the phosphopeptide derived from the corresponding region of ERK1&2 (7). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show that only the phosphopeptide corresponding to p38 [pTpY180/182] blocks the antibody signal, thereby demonstrating the specificity of the antibody.)
Testing Data
(Peptide Competition Extracts prepared from HEK 293 cells treated with UV irradiation were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSATBST buffer overnight at 4oC, then were incubated with p38 [pTpY180/182] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with: the peptide immunogen (1), a generic phosphothreonine-containing peptide (2), a generic phosphotyrosine-containing peptide (3), the non-phosphorylated peptide corresponding to the phosphopeptide (4), no peptide (5), the phosphopeptide derived from the corresponding region of JNK1&2 (6), and, the phosphopeptide derived from the corresponding region of ERK1&2 (7). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show that only the phosphopeptide corresponding to p38 [pTpY180/182] blocks the antibody signal, thereby demonstrating the specificity of the antibody.)
NCBI and Uniprot Product Information
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Product Notes
The MAP Kinase p38 (Catalog #AAA627818) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAP Kinase p38 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the MAP Kinase p38 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP Kinase p38, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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