SDS-Page
(Peptide Competition Extracts prepared from NIH3T3 cells left untreated (1) or treated with PDGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 4% BSA-TBST buffer for one hour at RT then were incubated with MEK1 [pT386] antibody in a 1% BSA-TBST buffer for two hours at room temperature, following prior incubation with: no peptide (1, 2), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphothreoninecontaining peptide (4), or the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show that only the peptide corresponding to MEK1 [pT386] blocks the antibody signal, thereby demonstrating the specificity of the antibody.)
Rabbit MEK1 Polyclonal Antibody | anti-MEK1 antibody
MEK1, phosphorylated (Thr386) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-Activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 1:1000
SDS-Page
(Peptide Competition Extracts prepared from NIH3T3 cells left untreated (1) or treated with PDGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 4% BSA-TBST buffer for one hour at RT then were incubated with MEK1 [pT386] antibody in a 1% BSA-TBST buffer for two hours at room temperature, following prior incubation with: no peptide (1, 2), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphothreoninecontaining peptide (4), or the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show that only the peptide corresponding to MEK1 [pT386] blocks the antibody signal, thereby demonstrating the specificity of the antibody.)
SDS-Page
(Peptide Competition Extracts prepared from NIH3T3 cells left untreated (1) or treated with PDGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 4% BSA-TBST buffer for one hour at RT then were incubated with MEK1 [pT386] antibody in a 1% BSA-TBST buffer for two hours at room temperature, following prior incubation with: no peptide (1, 2), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphothreoninecontaining peptide (4), or the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show that only the peptide corresponding to MEK1 [pT386] blocks the antibody signal, thereby demonstrating the specificity of the antibody.)
NCBI and Uniprot Product Information
NCBI Description
Member of MAP Kinase Kinase. Likely functions in a stress-activated MAPK pathway. Can phosphorylate the MAPK AtMPK4, in response to stress. Gets phosphorylated by MEKK1 in response to wounding.
Uniprot Description
Function: May be involved in the cold and salinity stress-mediated MAP kinase signaling cascade (MEKK1, MEK1/MKK2 and MPK4/MPK6). Phosphorylates MPK4 in vitro. Ref.8
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation: Activated through serine and threonine phosphorylation in response to wounding, cold, drought, salt stresses, hydrogen peroxide, bacterial flagellin and laminarin beta-glucan. Ref.10 Ref.12
Subunit structure: Interacts with MEKK1 (MAPKKK) and MPK4. May form a ternary complex with. Ref.7
Tissue specificity: Expressed in roots, stem, flowers and siliques. Ref.1
Induction: By wounding. Ref.1 Ref.10 Ref.12
Post-translational modification: Phosphorylated on Thr-218 and Ser-224, which activates the enzyme. Ref.9 Ref.10
Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Contains 1 protein kinase domain.
Research Articles on MEK1
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Product Notes
The MEK1 mkk1 (Catalog #AAA628480) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MEK1, phosphorylated (Thr386) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-Activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MEK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the MEK1 mkk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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