Testing Data
(Peptide Competition and Phosphatase Treatment: Lysates prepared from K562 cells stimulated with PMA were either left untreated (1-4) or treated with lambda (l) phosphatase (5), then incubated with PKCepsilon [pS729] antibody (2 hr, RT), following prior in)
Rabbit anti-Human, Mouse Protein Kinase C, epsilon Polyclonal Antibody | anti-PRKCE antibody
Protein Kinase C, epsilon, phosphorylated (Ser729) (PKCe)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 1:1000
Testing Data
(Peptide Competition and Phosphatase Treatment: Lysates prepared from K562 cells stimulated with PMA were either left untreated (1-4) or treated with lambda (l) phosphatase (5), then incubated with PKCepsilon [pS729] antibody (2 hr, RT), following prior in)
Testing Data
(Peptide Competition and Phosphatase Treatment: Lysates prepared from K562 cells stimulated with PMA were either left untreated (1-4) or treated with lambda (l) phosphatase (5), then incubated with PKCepsilon [pS729] antibody (2 hr, RT), following prior in)
Testing Data
(Anti-PKCε pan, ERK1&2 [pTpY185/187], Ιkka [pSpS176/180], Peptide Competition and Phosphatase Treatment Lysates prepared from K562 cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda (λ) phosphatase (5), blocked with a 3% BSA-TBST buffer for one hour at room temperature, and incubated with PKCε [pS729] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphoserine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to PKCε [pS729] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.)
Testing Data
(Anti-PKCε pan, ERK1&2 [pTpY185/187], Ιkka [pSpS176/180], Peptide Competition and Phosphatase Treatment Lysates prepared from K562 cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda (λ) phosphatase (5), blocked with a 3% BSA-TBST buffer for one hour at room temperature, and incubated with PKCε [pS729] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphoserine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to PKCε [pS729] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.)
NCBI and Uniprot Product Information
NCBI Description
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq]
Uniprot Description
PKCE: an AGC kinase of the PKC family. Calcium-independent, phospholipid-dependent. Activated by inflammatory mediators and involved in nociceptive functions. PKC-epsilon null mice display decreased hypoxic pulmonary vasoconstriction.
Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, AGC; EC 2.7.11.13; AGC group; PKC family; Eta subfamily
Chromosomal Location of Human Ortholog: 2p21
Cellular Component: Golgi apparatus; cytoskeleton; mitochondrion; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytosol; nucleus
Molecular Function: actin monomer binding; calcium-independent protein kinase C activity; protein serine/threonine kinase activity; signal transducer activity; protein binding; protein kinase C activity; enzyme binding; metal ion binding; enzyme activator activity; ethanol binding; ATP binding; receptor activator activity
Biological Process: nerve growth factor receptor signaling pathway; apoptosis; response to morphine; positive regulation of lipid catabolic process; signal transduction; protein amino acid phosphorylation; positive regulation of MAPKKK cascade; lipopolysaccharide-mediated signaling pathway; cell adhesion; regulation of peptidyl-tyrosine phosphorylation; epidermal growth factor receptor signaling pathway; positive regulation of cytokinesis; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; fibroblast growth factor receptor signaling pathway; positive regulation of synaptic transmission, GABAergic; positive regulation of insulin secretion; cell cycle; peptidyl-serine phosphorylation; positive regulation of actin filament polymerization; cell division; phospholipase C activation; release of sequestered calcium ion into cytosol; macrophage activation during immune response; innate immune response; blood coagulation
Research Articles on PRKCE
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Product Notes
The PRKCE prkce (Catalog #AAA628260) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Protein Kinase C, epsilon, phosphorylated (Ser729) (PKCe) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Protein Kinase C, epsilon can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the PRKCE prkce for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein Kinase C, epsilon, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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