SDS-Page
(Peptide Competition Extracts prepared from HEK293 cells left untreated (1) or treated with UV (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with STAT1 [pS727] antibody for two hours at room temperature in a 3% Milk-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRPconjugate and bands were detected using the Pierce SuperSignal method. The data show that only the peptide corresponding to STAT1 [pS727] blocks the antibody signal, demonstrating the specificity of the antibody.)
Rabbit anti-Human, Mouse STAT 1 Polyclonal Antibody | anti-SOAT1 antibody
STAT 1, phosphorylated (Ser727) (Signal Transducer and Activator of Transcription 1)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 1:1000
Optimal dilution to be determined by the researcher.
SDS-Page
(Peptide Competition Extracts prepared from HEK293 cells left untreated (1) or treated with UV (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with STAT1 [pS727] antibody for two hours at room temperature in a 3% Milk-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRPconjugate and bands were detected using the Pierce SuperSignal method. The data show that only the peptide corresponding to STAT1 [pS727] blocks the antibody signal, demonstrating the specificity of the antibody.)
SDS-Page
(Peptide Competition Extracts prepared from HEK293 cells left untreated (1) or treated with UV (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with STAT1 [pS727] antibody for two hours at room temperature in a 3% Milk-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRPconjugate and bands were detected using the Pierce SuperSignal method. The data show that only the peptide corresponding to STAT1 [pS727] blocks the antibody signal, demonstrating the specificity of the antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the acyltransferase family. It is located in the endoplasmic reticulum, and catalyzes the formation of fatty acid-cholesterol esters. This gene has been implicated in the formation of beta-amyloid and atherosclerotic plaques by controlling the equilibrium between free cholesterol and cytoplasmic cholesteryl esters. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2011]
Uniprot Description
SOAT1: Catalyzes the formation of fatty acid-cholesterol esters. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.
Protein type: Transferase; Membrane protein, integral; EC 2.3.1.26; Membrane protein, multi-pass; Lipid Metabolism - steroid biosynthesis
Chromosomal Location of Human Ortholog: 1q25
Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane
Molecular Function: protein binding; acyl-CoA binding; sterol O-acyltransferase activity; cholesterol binding
Biological Process: cholesterol metabolic process; cholesterol homeostasis; cholesterol efflux; positive regulation of amyloid precursor protein biosynthetic process
Research Articles on SOAT1
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Product Notes
The SOAT1 soat1 (Catalog #AAA628431) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The STAT 1, phosphorylated (Ser727) (Signal Transducer and Activator of Transcription 1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's STAT 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000 Optimal dilution to be determined by the researcher. Researchers should empirically determine the suitability of the SOAT1 soat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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