SDS-Page
(Peptide Competition Extracts prepared from PC12 cells left unstimulated (1) or stimulated with sorbitol (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with MBS622294 for two hours at RT in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphothreonine-containing peptide (4), a generic phosphotyrosine-containing peptide (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method.)
Rabbit anti-Human Erk1,2 Polyclonal Antibody
Erk1,2, phosphorylated (Thr185, Tyr187) (Extracellular Signal-regulated Kinase 1, ERK1, ERK-1, ERK, Extracellular Signal-regulated Kinase 2, ERK2, ERK-2, ERT1, ERT2, Insulin-stimulated MAP2 Kinase, MAP Kinase Isoform p42, MAP Kinase Isoform p44, Microtubu
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Erk1; 2; Polyclonal Antibody; phosphorylated (Thr185; Tyr187) (Extracellular Signal-regulated Kinase 1; ERK1; ERK-1; ERK; Extracellular Signal-regulated Kinase 2; ERK2; ERK-2; ERT1; ERT2; Insulin-stimulated MAP2 Kinase; MAP Kinase Isoform p42; MAP Kinase Isoform p44; Microtubu; Anti -Erk1; anti-Erk1; 2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ERK1 and ERK2 when phosphorylated at Thr185 and Tyr187. Species Sequence Homology: rat, mouse, bovine, frog, snail, nematode, and drosophila.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
Applicable Applications for anti-Erk1,2 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000
Dilution: Western Blot: 1:1000
Immunogen
Synthetic phosphopeptide corresponding to human ERK1 and 2 that contains threonine 202/185 and tyrosine 204/187.
Positive Control
PC12 cells +/- Sorbitol; NIH3T3 +/-PDGF; HEK2893+/-UV
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
SDS-Page
(Peptide Competition Extracts prepared from PC12 cells left unstimulated (1) or stimulated with sorbitol (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with MBS622294 for two hours at RT in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphothreonine-containing peptide (4), a generic phosphotyrosine-containing peptide (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method.)
SDS-Page
(Peptide Competition Extracts prepared from PC12 cells left unstimulated (1) or stimulated with sorbitol (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with MBS622294 for two hours at RT in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphothreonine-containing peptide (4), a generic phosphotyrosine-containing peptide (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method.)
Testing Data
(Peptide Competition: Extracts prepared from PC12 cells left unstimulated (1) or stimulated with sorbitol (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with MBS622294 for two hours at RT in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphothreonine-containing peptide (4), a generic phosphotyrosine-containing peptide (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to ERK1&2 [pTpY185/187] blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show the induction of phosphorylation by the addition of sorbitol.)
Testing Data
(Peptide Competition: Extracts prepared from PC12 cells left unstimulated (1) or stimulated with sorbitol (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with MBS622294 for two hours at RT in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphothreonine-containing peptide (4), a generic phosphotyrosine-containing peptide (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to ERK1&2 [pTpY185/187] blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show the induction of phosphorylation by the addition of sorbitol.)
Related Product Information for anti-Erk1,2 antibody
ERK (extracellular signal-regulated kinase), also known as MAPK (mitogen-activated protein kinase) has two closely related isoforms of 44kD and 42kD, respectively. These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with a large variety of stimuli including mitogens, hormones, growth factors, cytokines and bioactive peptides. Cell stimulation induces the activation of a signaling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as the cytoskeleton. ERK1 and ERK2 are fully activated by phosphorylation within a Thr-Glu-Tyr motif of the activation loop on both a threonine and a tyrosine residue by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of the ERK1 and 2 enzymes.
Product Categories/Family for anti-Erk1,2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Extracellular signal-regulated kinase 1
Similar Products
Product Notes
The Erk1,2 (Catalog #AAA622294) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Erk1,2, phosphorylated (Thr185, Tyr187) (Extracellular Signal-regulated Kinase 1, ERK1, ERK-1, ERK, Extracellular Signal-regulated Kinase 2, ERK2, ERK-2, ERT1, ERT2, Insulin-stimulated MAP2 Kinase, MAP Kinase Isoform p42, MAP Kinase Isoform p44, Microtubu reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Erk1,2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the Erk1,2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Erk1,2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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