Rabbit anti-Mouse Munc18-1 (Ser241) Polyclonal Antibody | anti-STXBP1 antibody

Anti-Phospho-Ser241 Munc18-1

Cat. Num. AAA502183

• Gene Names: Stxbp1; Ms10g; nsec1; N-sec1; Sxtbp1; Unc18h; MMS10-G; Rb-sec1; Unc18-1; AI317162; AI326233; Munc-18a; Munc18-1
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: Munc18-1 (Ser241); Polyclonal Antibody; Anti-Phospho-Ser241 Munc18-1; anti-STXBP1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: Specific for the ~65k Munc18-1 protein phosphorylated at Ser241. Immunolabeling is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein).
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 603

• Applicable Applications for anti-STXBP1 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho- Ser241 of mouse Munc18-1.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser241 conjugated to KLH
• Immunogen Species: Mouse
• Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, chicken, human, non-human primates, rat and sheep
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with mouse tissue. It is anticipated that the antibody will react with bovine, canine, human, non-human primates, rat, and sheep based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc18-1 is thought to be regulated by phosphorylation.
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-STXBP1 antibody

Affinity purified rabbit polyclonal antibody

References

• Ciufo LF, Barclay JW, Burgoyne RD, Morgan A (2005) Munc18-1 regulates early and late stages of exocytosis via syntaxin-independent protein interactions. Mol Biol Cell 16:470-482.
• Liu J, Ernst SA, Gladycheva SE, Lee YY, Lentz SI, Ho CS, Li Q, Stuenkel EL (2004) Fluorescence resonance energy transfer reports properties of syntaxin1a interaction with Munc18-1 in vivo. J Biol Chem 279:55924-55936.

NCBI and Uniprot Product Information

• NCBI GI #: 165972307
• NCBI GeneID: 20910
• NCBI Accession #: NP_001107041.1
• NCBI GenBank Nucleotide #: NM_001113569.1
• UniProt Accession #: O08599; Q5WAC6; Q8VD51; A2ARS2; A2ARS3; A2ARS4
• Molecular Weight: 65
• NCBI Official Full Name: syntaxin-binding protein 1 isoform a
• NCBI Official Synonym Full Names: syntaxin binding protein 1
• NCBI Official Symbol: Stxbp1
• NCBI Official Synonym Symbols: Ms10g; nsec1; N-sec1; Sxtbp1; Unc18h; MMS10-G; Rb-sec1; Unc18-1; AI317162; AI326233; Munc-18a; Munc18-1
• NCBI Protein Information: syntaxin-binding protein 1; unc-18A; unc-18-1; UNC-18 homolog; protein unc-18 homolog 1; protein unc-18 homolog A; minisatellite 10g detected by probe MMS10
• UniProt Protein Name: Syntaxin-binding protein 1
• Protein Family: Syntaxin-binding protein
• UniProt Gene Name: Stxbp1
• UniProt Synonym Gene Names: Unc18-1; Unc-18A
• UniProt Entry Name: STXB1_MOUSE

Uniprot Description

Function: May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions

By similarity.

Subunit structure: Binds SYTL4

By similarity. Interacts with STX1A

By similarity.

Sequence similarities: Belongs to the STXBP/unc-18/SEC1 family.

Sequence caution: The sequence CAM20726.1 differs from that shown. Reason: Erroneous gene model prediction.