Munc18-1/STXBP1 Recombinant Protein | STXBP1 recombinant protein

Munc18-1/STXBP1 Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8120963

• Gene Names: STXBP1; P67; NSEC1; UNC18; RBSEC1; MUNC18-1
• Purity: >85% as determined by SDS-PAGE
• Synonyms: Munc18-1/STXBP1; Munc18-1/STXBP1 Protein; Human; Recombinant (His & GST Tag); Human STXBP1/UNC18A Protein (His & GST Tag); MUNC18-1 Protein; NSEC1 Protein; P67 Protein; RBSEC1 Protein; UNC18 Protein; syntaxin binding protein 1; STXBP1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 0.5mM PMSF, 10% gly, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser594

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the human STXBP1 isoform 1 (P61764-1)(Met 1-Ser 594) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for STXBP1 recombinant protein

Background: Syntaxin-binding protein 1, also known as N-Sec1, Protein unc-18 homolog 1, MUNC18-1 and STXBP1, is a peripheral membrane protein that belongs to the STXBP/unc-18/SEC1 family. STXBP1 is an evolutionally conserved neuronal Sec1/Munc-18 (SM) protein that is essential in synaptic vesicle release in several species. It may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. STXBP1 is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. It can interact with syntaxins 1, 2, and 3 but not syntaxin 4. STXBP1 may also play a role in determining the specificity of intracellular fusion reactions. Defects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4). Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination.

References

Gengyo-Ando K., et al., 1996, J. Neurosci. 16: 6695-6702. Swanson D.A., et al., 1998, Genomics 48: 373-376. The MGC Project Team. 2004, Genome Res. 14: 2121-2127. Rush J., et al., 2005, Nat. Biotechnol. 23: 94-101. Saitsu H., et al., 2008, Nat. Genet. 40:782-788.

NCBI and Uniprot Product Information

• NCBI GI #: 73760415
• NCBI GeneID: 6812
• NCBI Accession #: NP_001027392.1
• NCBI GenBank Nucleotide #: NM_001032221.3
• UniProt Accession #: P61764; Q28208; Q62759; Q64320; Q96TG8; B1AM97
• Molecular Weight: 67,569 Da
• NCBI Official Full Name: syntaxin-binding protein 1 isoform b
• NCBI Official Synonym Full Names: syntaxin binding protein 1
• NCBI Official Symbol: STXBP1
• NCBI Official Synonym Symbols: P67; NSEC1; UNC18; RBSEC1; MUNC18-1
• NCBI Protein Information: syntaxin-binding protein 1; N-Sec1; unc-18A; unc18-1; neuronal SEC1; protein unc-18 homolog 1; protein unc-18 homolog A
• UniProt Protein Name: Syntaxin-binding protein 1
• Protein Family: Syntaxin-binding protein
• UniProt Gene Name: STXBP1
• UniProt Synonym Gene Names: UNC18A; Unc18-1; Unc-18A
• UniProt Entry Name: STXB1_HUMAN

NCBI Description

This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010]

Uniprot Description

STXBP1: may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. Two splice-variant isoforms have been described.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: nucleoplasm; protein complex; mitochondrion; cytoplasm; plasma membrane; cytosol

Molecular Function: identical protein binding; protein domain specific binding; SNARE binding; syntaxin binding; syntaxin-1 binding; protein N-terminus binding; protein kinase binding

Biological Process: protein stabilization; synaptic vesicle maturation; neurotransmitter secretion; regulation of synaptic vesicle fusion to presynaptic membrane; positive regulation of calcium ion-dependent exocytosis; synaptic transmission; protein transport; platelet degranulation; axon target recognition; glutamate secretion; energy reserve metabolic process; neuromuscular synaptic transmission; negative regulation of neuron apoptosis; negative regulation of synaptic transmission, GABAergic; regulation of insulin secretion; vesicle docking during exocytosis

Disease: Epileptic Encephalopathy, Early Infantile, 4