Rabbit MAP Kinase, p38 Polyclonal Antibody

MAP Kinase, p38, phosphorylated (Thr180/Tyr182) (MapK14, Mitogen-activated Protein Kinase p38 alpha, Mitogen-activated Protein Kinase 14, CRK1, CSBP1, CSBP2)

Cat. Num. AAA627039

• Reactivity: Drosophila, Human, Monkey, Mouse, Rat0
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Ascites; Ascites
• Synonyms: MAP Kinase; p38; Polyclonal Antibody; phosphorylated (Thr180/Tyr182) (MapK14; Mitogen-activated Protein Kinase p38 alpha; Mitogen-activated Protein Kinase 14; CRK1; CSBP1; CSBP2); Anti -MAP Kinase; anti-MAP Kinase; p38 antibody

• Host: Rabbit
• Reactivity: Drosophila, Human, Monkey, Mouse, Rat0
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of human p38 MAPK only when dually phosphorylated at threonine 180 and tyrosine182. Does not crossreact with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. Species Crossreactivity
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 01mg/ml BSA, <0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-MAP Kinase, p38 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.; Dilution: Western Blot: 1:1000. Iincubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20.; Immunohistochemistry (Paraffin): 1:50. Antigen retrieval: heating in citrate buffer. Dilute antibody in TBS-Tween plus 5% normal goat serum.; Immunofluorescence (IF-IC): 1:100; Optimal dilution determined by the researcher.
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAPK (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of extracts from Jurkat, NIH/3T3 and PC12 cells, untreated or treated as indicated, using MBS627039.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded lung carcinoma, showing nuclear localization, using MBS627039.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded breast carcinoma, using MBS627039 (left) or the same antibody preincubated with antigen phospho-peptide (right).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded NIH/3T3 cells, untreated (left) or anisomycin-treated (right), using MBS627039.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HeLa cells -/+ UV light, labeled with MBS627039 (green). Absence of staining in untreated cells (left) and nuclear localization in treated cells (right). Red = Actin filaments (phalloidin).)

Related Product Information for anti-MAP Kinase, p38 antibody

p38 MAP kinase (MAPK), also called RK or CSBP, is the mammalian orthologue of the yeast HOG kinase which participates in a signaling cascade controlling cellular responses to cytokines and stress. Four isoforms of p38 MAP kinase, p38a, b and g (also known as ERK6 or SAPK3) and delta (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides LPS), UV light and growth factors. MKK3, MKK6 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2, Max and MEF2.

Product Categories/Family for anti-MAP Kinase, p38 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 529040
• NCBI Official Full Name: MAP kinase