Western Blot (WB)
(Western Blot (WB) analysis of WT1-AS polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)
Rabbit WT1-AS Polyclonal Antibody | anti-WT1-AS antibody
WT1-AS Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
WT1-AS; Polyclonal Antibody; WT1-AS Polyclonal Antibody; Putative Wilms tumor upstream neighbor 1 gene protein; WIT-1; Wilms tumor-associated antisense RNA; WIT1; anti-WT1-AS antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
WT1-AS polyclonal antibody detects endogenous levels of WT1-AS protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
92
Applicable Applications for anti-WT1-AS antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human WT1-AS.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of WT1-AS polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of WT1-AS polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)
Related Product Information for anti-WT1-AS antibody
Wilms tumor is a neoplasm of the kidneys that usually occurs in children and is characterized by the presence of abortive tubules and glomeruli surrounded by a spindled cell stroma. The 11p13 Wilms tumor locus consists of two coordinately regulated transcripts, WT1 and WIT-1, which are mutated in Wilms tumors. Wilms tumor upstream neighbor 1 (WIT-1) is encoded by an intron-less gene upstream of the Wilms tumor 1 (WT1) gene, which is is important for nephrogenesis and gonadal growth. The WT1 gene is bi-directionally transcribed from the same promoter region as WIT-1, which may function as an antisense regulator of WT1. WIT-1 and WT1 have the same temporal and cell-restricted expression pattern, although the expression of WIT-1 is less abundant. Methylation of the WIT-1 gene is implicated in hematologic malignancy of chemoresistant acute myeloid leukemia. Single nucleotide polymorphisms (SNPs) in the WIT-1 gene are significantly associated with focal segmental glomerulosclerosis.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
~ 15kDa
NCBI Official Full Name
Putative Wilms tumor upstream neighbor 1 gene protein
UniProt Protein Name
Putative Wilms tumor upstream neighbor 1 gene protein
Protein Family
UniProt Gene Name
WT1-AS
UniProt Synonym Gene Names
WIT1; WIT-1
UniProt Entry Name
WIT1_HUMAN
Uniprot Description
WIT1:
Chromosomal Location of Human Ortholog: 11p13
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Product Notes
The WT1-AS wt1-as (Catalog #AAA3006340) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WT1-AS Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's WT1-AS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the WT1-AS wt1-as for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WT1-AS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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