Rabbit KPTN Polyclonal Antibody | anti-KPTN antibody

KPTN Antibody

Cat. Num. AAA9611866

• Gene Names: KPTN; 2E4; MRT41
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: KPTN; Polyclonal Antibody; KPTN Antibody; anti-KPTN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: KPTN Antibody detects endogenous levels of KPTN.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 436

• Applicable Applications for anti-KPTN antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human KPTN.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Lysosome
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using KPTN Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-KPTN antibody

Description: As part of the KICSTOR complex functions in the amino acid-sensing branch of the TORC1 signaling pathway. Recruits, in an amino acid-independent manner, the GATOR1 complex to the lysosomal membranes and allows its interaction with GATOR2 and the RAG GTPases. Functions upstream of the RAG GTPases and is required to negatively regulate mTORC1 signaling in absence of amino acids. In absence of the KICSTOR complex mTORC1 is constitutively localized to the lysosome and activated. The KICSTOR complex is also probably involved in the regulation of mTORC1 by glucose.

Subunit Structure: Part of the KICSTOR complex composed of KPTN, ITFG2, C12orf66 and SZT2. SZT2 probably serves as a link between the other three proteins in the KICSTOR complex and mediates the direct interaction with the GATOR1 complex (PubMed:28199306). May associate with F-actin filaments (PubMed:10099934).

NCBI and Uniprot Product Information

• NCBI GI #: 103471989
• NCBI GeneID: 11133
• NCBI Accession #: NP_008990.2
• NCBI GenBank Nucleotide #: NP_008990.2
• UniProt Accession #: Q9Y664
• Molecular Weight: Observed: 48 kDa; Predicted: 49 kDa
• NCBI Official Full Name: KICSTOR complex protein kaptin isoform 1
• NCBI Official Synonym Full Names: kaptin, actin binding protein
• NCBI Official Symbol: KPTN
• NCBI Official Synonym Symbols: 2E4; MRT41
• NCBI Protein Information: KICSTOR complex protein kaptin
• UniProt Protein Name: KICSTOR complex protein kaptin
• Protein Family: Kaptin
• UniProt Gene Name: KPTN

NCBI Description

This gene encodes a filamentous-actin-associated protein, which is involved in actin dynamics and plays an important role in neuromorphogenesis. This protein is part of the KICSTOR protein complex that localizes to lysosomes. Mutations in this gene result in an autosomal recessive form of intellectual disability. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2017]

Uniprot Description

As part of the KICSTOR complex functions in the amino acid-sensing branch of the TORC1 signaling pathway. Recruits, in an amino acid-independent manner, the GATOR1 complex to the lysosomal membranes and allows its interaction with GATOR2 and the RAG GTPases. Functions upstream of the RAG GTPases and is required to negatively regulate mTORC1 signaling in absence of amino acids. In absence of the KICSTOR complex mTORC1 is constitutively localized to the lysosome and activated. The KICSTOR complex is also probably involved in the regulation of mTORC1 by glucose.